naa a22 4500 510817335 CHVBK 20180411083514.0 cr unu---uuuuu 180411e20130601xx s 000 0 eng 10.1007/s11468-013-9538-6 doi (NATIONALLICENCE)springer-10.1007/s11468-013-9538-6 In Vitro Identification of Gold Nanorods through Hyperspectral Imaging [Elektronische Daten] [Bradley Stacy, Kristen Comfort, Donald Comfort, Saber Hussain] Due to their unique plasmonic and optical properties, gold nanorods (GNR) have shown tremendous potential for nano-based applications extending into a variety of fields including bioimaging, sensor development, electronics, and cancer therapy. These distinctive, shape-specific properties are strongly dependent upon the GNR aspect ratio, thus producing the ability to be targeted for an application by fine-tuning their physical parameters. It is owing to their characteristic spectral signature, which is vastly different from that of a cellular setting, that GNRs are emerging as an ideal candidate for nano-based imaging applications. However, one challenge that has emerged in the field of bioimaging is the need to account for the observed plasmon coupling effect that arises from GNR agglomeration in a physiological environment. In this study, GNRs with aspect ratios of 2.5 and 6.0 were actively identified in an in vitro setting through a hyperspectral imaging (HSI) analysis; which successfully recognized and separated the light scattering pattern of these particles from that of the surrounding cells. Through inclusion of agglomerated GNR spectral patterns in the HSI spectral library, this imaging technique was able to overcome the complication of plasmon coupling, though to varying degrees. These results demonstrate the tremendous potential of GNRs coupled with HSI analysis to advance the field of nano-based sensing and imaging mechanisms. Springer Science+Business Media New York, 2013 Gold nanorods nationallicence Hyperspectral imaging nationallicence Plasmonic resonance nationallicence Spectral signature nationallicence Agglomeration nationallicence In vitro identification nationallicence Stacy Bradley Applied Biotechnology Branch, Human Effectiveness Directorate, 711 Human Performance Wing, Air Force Research Laboratory, Wright-Patterson AFB, 45433, Dayton, OH, USA aut Comfort Kristen Applied Biotechnology Branch, Human Effectiveness Directorate, 711 Human Performance Wing, Air Force Research Laboratory, Wright-Patterson AFB, 45433, Dayton, OH, USA aut Comfort Donald Department of Chemical and Materials Engineering, University of Dayton, 45469, Dayton, OH, USA aut Hussain Saber Applied Biotechnology Branch, Human Effectiveness Directorate, 711 Human Performance Wing, Air Force Research Laboratory, Wright-Patterson AFB, 45433, Dayton, OH, USA aut Plasmonics Springer US; http://www.springer-ny.com 8/2(2013-06-01), 1235-1240 1557-1955 8:2<1235 2013 8 11468 https://doi.org/10.1007/s11468-013-9538-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11468-013-9538-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Stacy Bradley Applied Biotechnology Branch, Human Effectiveness Directorate, 711 Human Performance Wing, Air Force Research Laboratory, Wright-Patterson AFB, 45433, Dayton, OH, USA aut NATIONALLICENCE 700 1- Comfort Kristen Applied Biotechnology Branch, Human Effectiveness Directorate, 711 Human Performance Wing, Air Force Research Laboratory, Wright-Patterson AFB, 45433, Dayton, OH, USA aut NATIONALLICENCE 700 1- Comfort Donald Department of Chemical and Materials Engineering, University of Dayton, 45469, Dayton, OH, USA aut NATIONALLICENCE 700 1- Hussain Saber Applied Biotechnology Branch, Human Effectiveness Directorate, 711 Human Performance Wing, Air Force Research Laboratory, Wright-Patterson AFB, 45433, Dayton, OH, USA aut NATIONALLICENCE 773 0- Plasmonics Springer US; http://www.springer-ny.com 8/2(2013-06-01), 1235-1240 1557-1955 8:2<1235 2013 8 11468 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer