naa a22 4500 51081736X CHVBK 20180411083514.0 cr unu---uuuuu 180411e20130601xx s 000 0 eng 10.1007/s11468-012-9460-3 doi (NATIONALLICENCE)springer-10.1007/s11468-012-9460-3 Localized Surface Plasmon Resonance (LSPR) Biosensor for the Protein Detection [Elektronische Daten] [Maximilien Cottat, Néné Thioune, Ana-Maria Gabudean, Nathalie Lidgi-Guigui, Monica Focsan, Simion Astilean, Marc Lamy de la Chapelle] In this paper, we investigate the ability of the gold nanorods (GNRs) to detect some proteins and demonstrate their potential to be used as plasmonic nanobiosensors. The GNRs were synthesized by a two-step seed-mediated growth procedure at room temperature. Firstly, a seed solution of gold nanoparticles was synthesized in the presence of cetyltrimethylammonium bromide surfactant and, subsequently, incorporated with appropriate amount of silver nitrate and tetrachloroauric acid solutions to grow GNRs with average length of 50nm and diameter of 14nm. We study the interaction of GNRs with proteins whose molecular weight varies from 6.5 up to 75kDa. We investigate the resulting solutions by means of UV-vis absorption spectroscopy to determine the effect of the proteins characteristics on the shift of the localized surface plasmon resonance (LSPR). We show that for the case when proteins are in large excess compared to the GNRs concentration, whatever the protein is, the LSPR shift is constant and does not depend on the protein molecular weight. Moreover, we have been able to demonstrate that the sensitivity of such LSPR sensor is around 10-9M/nm on a concentration range from 10-10 to 10-8M. Some comparison with finite-difference time-domain simulations have also shown that the number of proteins adsorbed at the GNRs surface is around 40. Springer Science+Business Media New York, 2012 Localize surface plasmon resonance (LSPR) nationallicence Gold nanorods (GNRs) nationallicence Biosensor nationallicence Protein nationallicence Cottat Maximilien Université Paris 13, Sorbonne Paris Cité, Laboratoire CSPBAT, CNRS (UMR 7244), 74 rue Marcel Cachin, 93017, Bobigny, France aut Thioune Néné Université Paris 13, Sorbonne Paris Cité, Laboratoire CSPBAT, CNRS (UMR 7244), 74 rue Marcel Cachin, 93017, Bobigny, France aut Gabudean Ana-Maria Nanobiophotonics and Laser Microspectroscopy Center, Interdisciplinary Research Institute on Bio-Nano-Sciences, Faculty of Physics, Babes-Bolyai University, T. Laurian 42, 400271, Cluj-Napoca, Romania aut Lidgi-Guigui Nathalie Université Paris 13, Sorbonne Paris Cité, Laboratoire CSPBAT, CNRS (UMR 7244), 74 rue Marcel Cachin, 93017, Bobigny, France aut Focsan Monica Nanobiophotonics and Laser Microspectroscopy Center, Interdisciplinary Research Institute on Bio-Nano-Sciences, Faculty of Physics, Babes-Bolyai University, T. Laurian 42, 400271, Cluj-Napoca, Romania aut Astilean Simion Nanobiophotonics and Laser Microspectroscopy Center, Interdisciplinary Research Institute on Bio-Nano-Sciences, Faculty of Physics, Babes-Bolyai University, T. Laurian 42, 400271, Cluj-Napoca, Romania aut Lamy de la Chapelle Marc Université Paris 13, Sorbonne Paris Cité, Laboratoire CSPBAT, CNRS (UMR 7244), 74 rue Marcel Cachin, 93017, Bobigny, France aut Plasmonics Springer US; http://www.springer-ny.com 8/2(2013-06-01), 699-704 1557-1955 8:2<699 2013 8 11468 https://doi.org/10.1007/s11468-012-9460-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11468-012-9460-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cottat Maximilien Université Paris 13, Sorbonne Paris Cité, Laboratoire CSPBAT, CNRS (UMR 7244), 74 rue Marcel Cachin, 93017, Bobigny, France aut NATIONALLICENCE 700 1- Thioune Néné Université Paris 13, Sorbonne Paris Cité, Laboratoire CSPBAT, CNRS (UMR 7244), 74 rue Marcel Cachin, 93017, Bobigny, France aut NATIONALLICENCE 700 1- Gabudean Ana-Maria Nanobiophotonics and Laser Microspectroscopy Center, Interdisciplinary Research Institute on Bio-Nano-Sciences, Faculty of Physics, Babes-Bolyai University, T. Laurian 42, 400271, Cluj-Napoca, Romania aut NATIONALLICENCE 700 1- Lidgi-Guigui Nathalie Université Paris 13, Sorbonne Paris Cité, Laboratoire CSPBAT, CNRS (UMR 7244), 74 rue Marcel Cachin, 93017, Bobigny, France aut NATIONALLICENCE 700 1- Focsan Monica Nanobiophotonics and Laser Microspectroscopy Center, Interdisciplinary Research Institute on Bio-Nano-Sciences, Faculty of Physics, Babes-Bolyai University, T. Laurian 42, 400271, Cluj-Napoca, Romania aut NATIONALLICENCE 700 1- Astilean Simion Nanobiophotonics and Laser Microspectroscopy Center, Interdisciplinary Research Institute on Bio-Nano-Sciences, Faculty of Physics, Babes-Bolyai University, T. Laurian 42, 400271, Cluj-Napoca, Romania aut NATIONALLICENCE 700 1- Lamy de la Chapelle Marc Université Paris 13, Sorbonne Paris Cité, Laboratoire CSPBAT, CNRS (UMR 7244), 74 rue Marcel Cachin, 93017, Bobigny, France aut NATIONALLICENCE 773 0- Plasmonics Springer US; http://www.springer-ny.com 8/2(2013-06-01), 699-704 1557-1955 8:2<699 2013 8 11468 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer