naa a22 4500 510817513 CHVBK 20180411083515.0 cr unu---uuuuu 180411e20130601xx s 000 0 eng 10.1007/s11468-012-9390-0 doi (NATIONALLICENCE)springer-10.1007/s11468-012-9390-0 A Highly Sensitive Enzyme Catalytic Method for the Detection of Ethanol Based on Resonance Scattering Effect of Gold Particles [Elektronische Daten] [Yanghe Luo, Pengfei Wang, Tingsheng Li, Jianniao Tian, Aihui Liang, Zhiliang Jiang] In pH8.4 Tris-HCl buffer solutions, alcohol dehydrogenase catalyzed the reaction between ethanol and nicotinamide adenine dinucleotide to produce acetaldehyde. In the medium of HCl, acetaldehyde reduced HAuCl4 to form gold particles that exhibited a strong resonance scattering (RS) peak at 600nm. The RS peak increased with ethanol concentration. The increased RS intensity at 600nm (ΔI 600nm) was proportional to the ethanol concentration (C) from 0.068 to 10.2mmol/L, with a regression equation of ΔI 600nm = 35.59 C + 16.1, and a detection limit (3σ) of 3.2μmol/L. This proposed method was applied to detect ethanol in saliva and plant cell culture medium samples, with satisfactory results. Springer Science+Business Media, LLC, 2012 Ethanol nationallicence Gold particle nationallicence Enzyme catalytic resonance scattering assay nationallicence Luo Yanghe Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education; Guangxi Key Laboratory of Environmental Engineering, Protection and Assessment, Guangxi Normal University, 541004, Guilin, China aut Wang Pengfei Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education; Guangxi Key Laboratory of Environmental Engineering, Protection and Assessment, Guangxi Normal University, 541004, Guilin, China aut Li Tingsheng Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education; Guangxi Key Laboratory of Environmental Engineering, Protection and Assessment, Guangxi Normal University, 541004, Guilin, China aut Tian Jianniao Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education; Guangxi Key Laboratory of Environmental Engineering, Protection and Assessment, Guangxi Normal University, 541004, Guilin, China aut Liang Aihui Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education; Guangxi Key Laboratory of Environmental Engineering, Protection and Assessment, Guangxi Normal University, 541004, Guilin, China aut Jiang Zhiliang Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education; Guangxi Key Laboratory of Environmental Engineering, Protection and Assessment, Guangxi Normal University, 541004, Guilin, China aut Plasmonics Springer US; http://www.springer-ny.com 8/2(2013-06-01), 307-312 1557-1955 8:2<307 2013 8 11468 https://doi.org/10.1007/s11468-012-9390-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11468-012-9390-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Luo Yanghe Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education; Guangxi Key Laboratory of Environmental Engineering, Protection and Assessment, Guangxi Normal University, 541004, Guilin, China aut NATIONALLICENCE 700 1- Wang Pengfei Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education; Guangxi Key Laboratory of Environmental Engineering, Protection and Assessment, Guangxi Normal University, 541004, Guilin, China aut NATIONALLICENCE 700 1- Li Tingsheng Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education; Guangxi Key Laboratory of Environmental Engineering, Protection and Assessment, Guangxi Normal University, 541004, Guilin, China aut NATIONALLICENCE 700 1- Tian Jianniao Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education; Guangxi Key Laboratory of Environmental Engineering, Protection and Assessment, Guangxi Normal University, 541004, Guilin, China aut NATIONALLICENCE 700 1- Liang Aihui Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education; Guangxi Key Laboratory of Environmental Engineering, Protection and Assessment, Guangxi Normal University, 541004, Guilin, China aut NATIONALLICENCE 700 1- Jiang Zhiliang Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education; Guangxi Key Laboratory of Environmental Engineering, Protection and Assessment, Guangxi Normal University, 541004, Guilin, China aut NATIONALLICENCE 773 0- Plasmonics Springer US; http://www.springer-ny.com 8/2(2013-06-01), 307-312 1557-1955 8:2<307 2013 8 11468 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer