naa a22 4500 510818323 CHVBK 20180411083518.0 cr unu---uuuuu 180411e20130601xx s 000 0 eng 10.1007/s12306-013-0264-1 doi (NATIONALLICENCE)springer-10.1007/s12306-013-0264-1 Metalloproteases and their inhibitors are altered in both torn and intact rotator cuff tendons [Elektronische Daten] [A. Castagna, E. Cesari, A. Gigante, M. Conti, R. Garofalo] Background: We evaluated the role of matrix metalloproteases (MMPs) and their inhibitors which are involved in extracellular matrix remodeling and degradation, in the pathogenesis of chronic rotator cuff tears. Materials and Methods: Tendon samples were harvested from 13 patients who underwent arthroscopic repair of a rotator cuff tear. Supraspinatus biopsy specimens were harvested en bloc from the arthroscopically intact middle portion of the tendon more than 1 cm from the torn edge, from the lateral edge of the tear, and from the superior one third of the macroscopically intact subscapularis tendon used as control. Histological analysis and an evaluation of the activity of specific metalloproteases and the tissue inhibitors of metalloprotease (TIMP-1, TIMP-2) was done blindly by multiplex sandwich ELISA (Search-Light technology) in each specimen Results: Histological evidence of tendinopathy was present in all patients with a tear of the rotator cuff, and not in the macroscopically intact subscapularis tendon. There was a significant increase in MMP 1, MMP 2, MMP 3 and in TIMP-1, TIMP-2 levels in all specimens examined, including the macroscopically intact portion of the supraspinatus tendon and in the control specimens Conclusions: The tissue in the ruptured area of the supraspinatus tendon undergoes marked rearrangement at molecular levels. This involves the activity of MMP 1, 2 and 3, and supports the critical role of MMPs in the tendon physiology. Seemingly intact parts of the injured supraspinatus tendon can present tendinopathic features, with altered cellular metabolism Istituto Ortopedico Rizzoli, 2013 Rotator cuff nationallicence Metalloproteinases nationallicence Shoulder nationallicence Tendon nationallicence Biology nationallicence Tear nationallicence Degenerative nationallicence Castagna A. Unita' Chirurgia Spalla e gomito, IRCCS Istituto Humanitas, Milan, Italy aut Cesari E. Unita' Chirurgia Spalla e gomito, IRCCS Istituto Humanitas, Milan, Italy aut Gigante A. Clinica di patologia dell'apparato locomotore, Università di Ancona, Ancona, Italy aut Conti M. Unita' Chirurgia Spalla e gomito, IRCCS Istituto Humanitas, Milan, Italy aut Garofalo R. Orthopedic Unit Miulli Hospital, Strada prov.le per Santeramo Km 4, Acquaviva delle fonti, BA, Italy aut MUSCULOSKELETAL SURGERY Springer Milan 97(2013-06-01), 39-47 2035-5106 97<39 2013 97 12306 https://doi.org/10.1007/s12306-013-0264-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12306-013-0264-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Castagna A. Unita' Chirurgia Spalla e gomito, IRCCS Istituto Humanitas, Milan, Italy aut NATIONALLICENCE 700 1- Cesari E. Unita' Chirurgia Spalla e gomito, IRCCS Istituto Humanitas, Milan, Italy aut NATIONALLICENCE 700 1- Gigante A. Clinica di patologia dell'apparato locomotore, Università di Ancona, Ancona, Italy aut NATIONALLICENCE 700 1- Conti M. Unita' Chirurgia Spalla e gomito, IRCCS Istituto Humanitas, Milan, Italy aut NATIONALLICENCE 700 1- Garofalo R. Orthopedic Unit Miulli Hospital, Strada prov.le per Santeramo Km 4, Acquaviva delle fonti, BA, Italy aut NATIONALLICENCE 773 0- MUSCULOSKELETAL SURGERY Springer Milan 97(2013-06-01), 39-47 2035-5106 97<39 2013 97 12306 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer