naa a22 4500 510818978 CHVBK 20180411083521.0 cr unu---uuuuu 180411e20130101xx s 000 0 eng 10.1007/s11814-012-0093-1 doi (NATIONALLICENCE)springer-10.1007/s11814-012-0093-1 Bioconversion process for synthesis of tert -butyl (3R,5S)-6-chloro-3,5-dihydroxyhexanoate using liquid-core immobilized Saccharomyces cerevisiae CGMCC No 2233 [Elektronische Daten] [Sun Xingyuan, Shi Hanbing, Bi Hongxia, Ou Zhimin] Tert-butyl (3R,5S)-6-chloro-3,5-dihydroxyhexanoate was synthesized using asymmetric reduction of tert-butyl (S)-6-chloro-5-hydroxy-3-oxo-hexanoate with liquid-core immobilized Saccharomyces cerevisiae CGMCC No. 2233. The optimum conditions for preparation of the liquid-core immobilized cells were found to be 2% guar gum, 5% CaCl2, 0.8% sodium alginate, capsule diameter 2mm, 0.3% chitosan (1.0×105) solution, and 30 min for formation of the film of liquid-core immobilized cells. The optimum re-cultivation time was 32 h. The optimum reduction conditions were found to be pH 6.8-7.2, 160 r/min, and 30 °C. Conversion was found to reach 100% when initial concentration of substrate was less than 50 g/L. The diastereomeric excess of tert-butyl (3R,5S)-6-chloro-3,5-dihydroxyhexanoate exceeded 99%. The liquid-core immobilized cells retained their effectiveness even after 15 uses. Korean Institute of Chemical Engineers, Seoul, Korea, 2012 Asymmetric Reduction nationallicence Tert -butyl (3R,5S)-6-chloro-3,5-dihydroxyhexanoate nationallicence Liquid-core Immobilized Cells nationallicence Xingyuan Sun The Third Affiliated Hospital of Qiqihar Medical College, 161000, Qiqihar, Heilongjiang, China aut Hanbing Shi The Third Affiliated Hospital of Qiqihar Medical College, 161000, Qiqihar, Heilongjiang, China aut Hongxia Bi The Third Affiliated Hospital of Qiqihar Medical College, 161000, Qiqihar, Heilongjiang, China aut Zhimin Ou Pharmaceuticals College, Zhejiang University of Technology, 310014, Hang Zhou, Zhejiang, China aut Korean Journal of Chemical Engineering Springer US; http://www.springer-ny.com 30/1(2013-01-01), 166-171 0256-1115 30:1<166 2013 30 11814 https://doi.org/10.1007/s11814-012-0093-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11814-012-0093-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xingyuan Sun The Third Affiliated Hospital of Qiqihar Medical College, 161000, Qiqihar, Heilongjiang, China aut NATIONALLICENCE 700 1- Hanbing Shi The Third Affiliated Hospital of Qiqihar Medical College, 161000, Qiqihar, Heilongjiang, China aut NATIONALLICENCE 700 1- Hongxia Bi The Third Affiliated Hospital of Qiqihar Medical College, 161000, Qiqihar, Heilongjiang, China aut NATIONALLICENCE 700 1- Zhimin Ou Pharmaceuticals College, Zhejiang University of Technology, 310014, Hang Zhou, Zhejiang, China aut NATIONALLICENCE 773 0- Korean Journal of Chemical Engineering Springer US; http://www.springer-ny.com 30/1(2013-01-01), 166-171 0256-1115 30:1<166 2013 30 11814 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer