naa a22 4500 510819281 CHVBK 20180411083523.0 cr unu---uuuuu 180411e20130601xx s 000 0 eng 10.1007/s11814-013-0058-z doi (NATIONALLICENCE)springer-10.1007/s11814-013-0058-z Co-immobilization of Candida rugosa and Rhyzopus oryzae lipases and biodiesel production [Elektronische Daten] [Jong Lee, Sung Kim, Hah Yoo, Ja Lee, Sung Han, Chulhwan Park, Seung Kim] Candida rugosa lipase and Ryzopus oryzae lipase were simultaneously immobilized on silica gel following enzyme pretreatment. The factors affecting the co-immobilization process, such as reaction time and enzyme ratio, were investigated. Biodiesel was then produced by using the co-immobilized enzyme matrix. A batch system was employed with stepwise methanol feeding, and the continuous process involved a packed-bed reactor. Under optimal immobilization conditions, the activity was approximately 16,000 U/g·matrix. When co-immobilized enzyme was used with optimized stepwise methanol feeding, conversion of biodiesel reached about 99% at 3 h and was maintained at a level of over 90% for about 30 reuses. Korean Institute of Chemical Engineers, Seoul, Korea, 2013 Biodiesel nationallicence Enzymatic Process nationallicence Co-immobilization nationallicence Lipase nationallicence Lee Jong Department of Chemical and Biological Engineering, Korea University, 5-1, Anam-dong, Sungbuk-gu, 136-701, Seoul, Korea aut Kim Sung Department of Chemical and Biological Engineering, Korea University, 5-1, Anam-dong, Sungbuk-gu, 136-701, Seoul, Korea aut Yoo Hah Department of Chemical and Biological Engineering, Korea University, 5-1, Anam-dong, Sungbuk-gu, 136-701, Seoul, Korea aut Lee Ja Department of Chemical and Biological Engineering, Korea University, 5-1, Anam-dong, Sungbuk-gu, 136-701, Seoul, Korea aut Han Sung College of Life Science and Biotechnology, Korea University, 5-1, Anam-dong, Sungbuk-gu, 136-701, Seoul, Korea aut Park Chulhwan Department of Chemical Engineering, Kwangwoon University, 447-1, Wolgye-dong, Nowon-gu, 139-701, Seoul, Korea aut Kim Seung Department of Chemical and Biological Engineering, Korea University, 5-1, Anam-dong, Sungbuk-gu, 136-701, Seoul, Korea aut Korean Journal of Chemical Engineering Springer US; http://www.springer-ny.com 30/6(2013-06-01), 1335-1338 0256-1115 30:6<1335 2013 30 11814 https://doi.org/10.1007/s11814-013-0058-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11814-013-0058-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lee Jong Department of Chemical and Biological Engineering, Korea University, 5-1, Anam-dong, Sungbuk-gu, 136-701, Seoul, Korea aut NATIONALLICENCE 700 1- Kim Sung Department of Chemical and Biological Engineering, Korea University, 5-1, Anam-dong, Sungbuk-gu, 136-701, Seoul, Korea aut NATIONALLICENCE 700 1- Yoo Hah Department of Chemical and Biological Engineering, Korea University, 5-1, Anam-dong, Sungbuk-gu, 136-701, Seoul, Korea aut NATIONALLICENCE 700 1- Lee Ja Department of Chemical and Biological Engineering, Korea University, 5-1, Anam-dong, Sungbuk-gu, 136-701, Seoul, Korea aut NATIONALLICENCE 700 1- Han Sung College of Life Science and Biotechnology, Korea University, 5-1, Anam-dong, Sungbuk-gu, 136-701, Seoul, Korea aut NATIONALLICENCE 700 1- Park Chulhwan Department of Chemical Engineering, Kwangwoon University, 447-1, Wolgye-dong, Nowon-gu, 139-701, Seoul, Korea aut NATIONALLICENCE 700 1- Kim Seung Department of Chemical and Biological Engineering, Korea University, 5-1, Anam-dong, Sungbuk-gu, 136-701, Seoul, Korea aut NATIONALLICENCE 773 0- Korean Journal of Chemical Engineering Springer US; http://www.springer-ny.com 30/6(2013-06-01), 1335-1338 0256-1115 30:6<1335 2013 30 11814 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer