naa a22 4500 510819508 CHVBK 20180411083524.0 cr unu---uuuuu 180411e20130901xx s 000 0 eng 10.1007/s11814-013-0108-6 doi (NATIONALLICENCE)springer-10.1007/s11814-013-0108-6 Anti-inflammatory effects of silkworm hemolymph on lipopolysaccharide-stimulated macrophages [Elektronische Daten] [Mi-Ra Chang, Woong Lee, Won Rhee, Tai Park, Eun Kim] Macrophages participate in several inflammatory pathologies such as sepsis and arthritis. We investigated the effect of silkworm hemolymph (SH) on the LPS-induced pro-inflammatory macrophages. SH inhibits LPS-induced nitric oxide (NO) production in RAW 264.7 cells and murine peritoneal macrophages. The decreased NO was reflected as a decreased amount of inducible nitric oxide synthase (iNOS) mRNA and protein. It was also found that SH inhibited pro-inflammatory cytokines, IL-1β, IL-6, and TNF-α production. To elucidate the mechanism by which SH inhibits NO production and iNOS expression, we investigated that SH suppressed IκB phosphorylation, which leads to the activation of NF-κB followed by degradation of IκB. This observation suggests that SH is a potential therapeutic modulator for inflammation-associated disorders. Korean Institute of Chemical Engineers, Seoul, Korea, 2013 Macrophages nationallicence Silkworm Hemolymph nationallicence Nitric Oxide (NO) nationallicence Inducible Nitric Oxide Synthase (iNOS) nationallicence Pro-inflammatory Cytokines nationallicence Chang Mi-Ra Division of Metrology for Quality Life, Korea Research Institute of Standards and Science, Doryong-dong, Yuseong-gu, 305-340, Daejeon, Korea aut Lee Woong Department of Dermatology, Seoul National University College of Medicine, 28, Yeongeon-dong, Jongno-gu, 110-744, Seoul, Korea aut Rhee Won Division of Bioengineering, Incheon National University, 119, Academy-ro, Yeonsu-gu, 406-772, Incheon, Korea aut Park Tai School of Chemical and Biological Engineering, Bio-MAX Institute, Seoul National University, 1, Gwanak-ro, Gwanak-gu, 151-744, Seoul, Korea aut Kim Eun Division of Metrology for Quality Life, Korea Research Institute of Standards and Science, Doryong-dong, Yuseong-gu, 305-340, Daejeon, Korea aut Korean Journal of Chemical Engineering Springer US; http://www.springer-ny.com 30/9(2013-09-01), 1784-1789 0256-1115 30:9<1784 2013 30 11814 https://doi.org/10.1007/s11814-013-0108-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11814-013-0108-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chang Mi-Ra Division of Metrology for Quality Life, Korea Research Institute of Standards and Science, Doryong-dong, Yuseong-gu, 305-340, Daejeon, Korea aut NATIONALLICENCE 700 1- Lee Woong Department of Dermatology, Seoul National University College of Medicine, 28, Yeongeon-dong, Jongno-gu, 110-744, Seoul, Korea aut NATIONALLICENCE 700 1- Rhee Won Division of Bioengineering, Incheon National University, 119, Academy-ro, Yeonsu-gu, 406-772, Incheon, Korea aut NATIONALLICENCE 700 1- Park Tai School of Chemical and Biological Engineering, Bio-MAX Institute, Seoul National University, 1, Gwanak-ro, Gwanak-gu, 151-744, Seoul, Korea aut NATIONALLICENCE 700 1- Kim Eun Division of Metrology for Quality Life, Korea Research Institute of Standards and Science, Doryong-dong, Yuseong-gu, 305-340, Daejeon, Korea aut NATIONALLICENCE 773 0- Korean Journal of Chemical Engineering Springer US; http://www.springer-ny.com 30/9(2013-09-01), 1784-1789 0256-1115 30:9<1784 2013 30 11814 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer