naa a22 4500 510819532 CHVBK 20180411083524.0 cr unu---uuuuu 180411e20130901xx s 000 0 eng 10.1007/s11814-013-0104-x doi (NATIONALLICENCE)springer-10.1007/s11814-013-0104-x Chitosan effects on glass matrices evaluated by biomaterial. MAS-NMR and biological investigations [Elektronische Daten] [Hassane Oudadesse, Eric Wers, Xuang Bui, Claire Roiland, Bruno Bureau, Imane Akhiyat, Amany Mostafa, Hassan Chaair, Hicham Benhayoune, Joel Fauré, Pascal Pellen-Mussi] Bioactive glass 46S6 and biodegradable therapeutic polymer (Chitosan: CH) have been elaborated to form 46S6-CH composite by freeze-drying process. The kinetics of chemical reactivity and bioactivity at the surface were investigated by using physicochemical techniques, particularly solid-state MAS-NMR. Immortalized cell line used to construct multicellular spheroids was employed as three-dimensional (3D) cell cultures for in vitro studies. Obtained results showed a novel structure of the composite; the chemical treatment (ultrasound, magnetic stirring, freeze drying process and lyophilization) led the bioactive glass particles to be loaded in the chitosan-based materials. 29Si and 31P MAS-NMR results showed the emergence of two new species, Q Si 3 (OH) and Q Si 4 , which are characteristic of the vitreous network dissolution in simulated body fluid (SBF). MAS-NMR also confirmed the formation of amorphous calcium phosphate (ACP) at the surface of the initial 46S6-CH. Three-dimensional (3D) cell cultures highlighted the effect of chitosan, where the cell viability reached up to 78% in 46S6-CH composite and up to 67% in 46S6. The association of (CH) and bioactive glass (BG) matrix promotes a highly significant bioactivity, demonstrating surface bone formation and satisfactory behavior in biological environment. Korean Institute of Chemical Engineers, Seoul, Korea, 2013 Bioactive Glass nationallicence Chitosan nationallicence Composite nationallicence Bioactivity nationallicence Hydroxyapatite nationallicence 31P MAS NMR nationallicence 29Si MAS NMR nationallicence Immortalized Cell nationallicence 3D Cells Viability nationallicence Oudadesse Hassane SCR, UMR CNRS 6226, University of Rennes 1, 263 av. du Général Leclerc, 35042, Rennes, France aut Wers Eric SCR, UMR CNRS 6226, University of Rennes 1, 263 av. du Général Leclerc, 35042, Rennes, France aut Bui Xuang SCR, UMR CNRS 6226, University of Rennes 1, 263 av. du Général Leclerc, 35042, Rennes, France aut Roiland Claire SCR, UMR CNRS 6226, University of Rennes 1, 263 av. du Général Leclerc, 35042, Rennes, France aut Bureau Bruno SCR, UMR CNRS 6226, University of Rennes 1, 263 av. du Général Leclerc, 35042, Rennes, France aut Akhiyat Imane SCR, UMR CNRS 6226, University of Rennes 1, 263 av. du Général Leclerc, 35042, Rennes, France aut Mostafa Amany SCR, UMR CNRS 6226, University of Rennes 1, 263 av. du Général Leclerc, 35042, Rennes, France aut Chaair Hassan Faculté des Sciences et Techniques de Mohammedia (FSTM), Université Hassan II, Maroc, France aut Benhayoune Hicham LISM-URCA, 21 rue Clément Ader, BP 138, 51685, Reims Cedex 02, France aut Fauré Joel LISM-URCA, 21 rue Clément Ader, BP 138, 51685, Reims Cedex 02, France aut Pellen-Mussi Pascal SCR, UMR CNRS 6226, University of Rennes 1, 263 av. du Général Leclerc, 35042, Rennes, France aut Korean Journal of Chemical Engineering Springer US; http://www.springer-ny.com 30/9(2013-09-01), 1775-1783 0256-1115 30:9<1775 2013 30 11814 https://doi.org/10.1007/s11814-013-0104-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11814-013-0104-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Oudadesse Hassane SCR, UMR CNRS 6226, University of Rennes 1, 263 av. du Général Leclerc, 35042, Rennes, France aut NATIONALLICENCE 700 1- Wers Eric SCR, UMR CNRS 6226, University of Rennes 1, 263 av. du Général Leclerc, 35042, Rennes, France aut NATIONALLICENCE 700 1- Bui Xuang SCR, UMR CNRS 6226, University of Rennes 1, 263 av. du Général Leclerc, 35042, Rennes, France aut NATIONALLICENCE 700 1- Roiland Claire SCR, UMR CNRS 6226, University of Rennes 1, 263 av. du Général Leclerc, 35042, Rennes, France aut NATIONALLICENCE 700 1- Bureau Bruno SCR, UMR CNRS 6226, University of Rennes 1, 263 av. du Général Leclerc, 35042, Rennes, France aut NATIONALLICENCE 700 1- Akhiyat Imane SCR, UMR CNRS 6226, University of Rennes 1, 263 av. du Général Leclerc, 35042, Rennes, France aut NATIONALLICENCE 700 1- Mostafa Amany SCR, UMR CNRS 6226, University of Rennes 1, 263 av. du Général Leclerc, 35042, Rennes, France aut NATIONALLICENCE 700 1- Chaair Hassan Faculté des Sciences et Techniques de Mohammedia (FSTM), Université Hassan II, Maroc, France aut NATIONALLICENCE 700 1- Benhayoune Hicham LISM-URCA, 21 rue Clément Ader, BP 138, 51685, Reims Cedex 02, France aut NATIONALLICENCE 700 1- Fauré Joel LISM-URCA, 21 rue Clément Ader, BP 138, 51685, Reims Cedex 02, France aut NATIONALLICENCE 700 1- Pellen-Mussi Pascal SCR, UMR CNRS 6226, University of Rennes 1, 263 av. du Général Leclerc, 35042, Rennes, France aut NATIONALLICENCE 773 0- Korean Journal of Chemical Engineering Springer US; http://www.springer-ny.com 30/9(2013-09-01), 1775-1783 0256-1115 30:9<1775 2013 30 11814 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer