naa a22 4500 51081994X CHVBK 20180411083526.0 cr unu---uuuuu 180411e20131201xx s 000 0 eng 10.1007/s11814-013-0190-9 doi (NATIONALLICENCE)springer-10.1007/s11814-013-0190-9 Production of polyhydroxyalkanoates by Ralstonia eutropha from volatile fatty acids [Elektronische Daten] [Jung Yun, Shailesh Sawant, Beom Kim] Polyhydroxyalkanoates (PHAs) are biodegradable and biocompatible thermoplastics that can be synthesized in various microorganisms. Volatile fatty acids (VFAs) are produced by anaerobic treatment of organic wastes that can be utilized as inexpensive substrates for PHA synthesis. In this study, several Ralstonia eutropha strains were grown on the mixture of VFAs (acetic, propionic, and butyric acid) as its carbon and energy source for growth and PHA synthesis. R. eutropha KCTC 2658 accumulated PHAs up to 50% of dry cell weight from total 5 g/L of mixed VFAs (acetic acid: propionic acid: butyric acid=1: 2: 2). In batch culture of R. eutropha KCTC2658 in a 5 L fermentor, a homopolymer of poly(3-hydroxybutyrate) [P(3HB)] was produced from 20 g/L glucose as a sole carbon source with dry cell weight of 8.4 g/L and PHA content of 30%. In fed-batch culture, two feeding strategies, pulse or pH-stat, were applied to add VFAs to the fermentor. When VFAs were fed using pH-stat feeding strategy after 40 h, a copolymer of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] was produced with dry cell weight of 8.1 g/L, PHA content of 50%, and 3HV fraction of 20 mol%. Korean Institute of Chemical Engineers, Seoul, Korea, 2013 Polyhydroxyalkanoates nationallicence PHA nationallicence Ralstonia eutropha nationallicence Volatile Fatty Acids nationallicence Yun Jung Department of Chemical Engineering, Chungbuk National University, 361-763, Cheongju, Chungbuk, Korea aut Sawant Shailesh Department of Chemical Engineering, Chungbuk National University, 361-763, Cheongju, Chungbuk, Korea aut Kim Beom Department of Chemical Engineering, Chungbuk National University, 361-763, Cheongju, Chungbuk, Korea aut Korean Journal of Chemical Engineering Springer US; http://www.springer-ny.com 30/12(2013-12-01), 2223-2227 0256-1115 30:12<2223 2013 30 11814 https://doi.org/10.1007/s11814-013-0190-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11814-013-0190-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yun Jung Department of Chemical Engineering, Chungbuk National University, 361-763, Cheongju, Chungbuk, Korea aut NATIONALLICENCE 700 1- Sawant Shailesh Department of Chemical Engineering, Chungbuk National University, 361-763, Cheongju, Chungbuk, Korea aut NATIONALLICENCE 700 1- Kim Beom Department of Chemical Engineering, Chungbuk National University, 361-763, Cheongju, Chungbuk, Korea aut NATIONALLICENCE 773 0- Korean Journal of Chemical Engineering Springer US; http://www.springer-ny.com 30/12(2013-12-01), 2223-2227 0256-1115 30:12<2223 2013 30 11814 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer