naa a22 4500 510820131 CHVBK 20180411083527.0 cr unu---uuuuu 180411e20130301xx s 000 0 eng 10.1007/s11814-012-0172-3 doi (NATIONALLICENCE)springer-10.1007/s11814-012-0172-3 Dispersion stability of citrate- and PVP-AgNPs in biological media for cytotoxicity test [Elektronische Daten] [Jinkyu Roh, Ha Umh, Jaehoon Sim, Sumin Park, Jongheop Yi, Younghun Kim] Silver nanoparticles (AgNPs) with antimicrobial property have been used for years in various applications, such as antibacterial coating and air/water purification to eliminate microorganisms. In recent years, a number of studies on the potential risk of nanomaterials in humans and the environment have appeared, and thus a large number of studies regarding the toxic effects of AgNPs on cells and micro-organisms have been reported. In in-vivo and in-vitro cytotoxicity tests, various biological media are used, and thus the dispersion stability of AgNPs in media is important to a successful toxicity test. Therefore, we investigated the dispersion stability of AgNPs in various biological media; PBS (phosphate buffered saline), FBS (fetal bovine serum), and de-ionized water (pH 2, 7, and 9) with and without photo-irradiation. Herein, citrate and PVP (polyvinylpyrrolidone) stabilized AgNPs were used as target materials. In short- and long-term stability tests, we found that PVP-AgNPs stabilized by steric hindrance have good dispersion stability in biological media, compared to citrate-AgNPs stabilized by electrostatic repulsion. Korean Institute of Chemical Engineers, Seoul, Korea, 2012 Silver Nanoparticles nationallicence Cytotoxicity nationallicence Dispersion Stability nationallicence Citrate nationallicence PVP nationallicence Roh Jinkyu Department of Chemical Engineering, Kwangwoon University, 139-701, Seoul, Korea aut Umh Ha Department of Chemical Engineering, Kwangwoon University, 139-701, Seoul, Korea aut Sim Jaehoon Department of Chemical Engineering, Kwangwoon University, 139-701, Seoul, Korea aut Park Sumin School of Chemical and Biological Engineering, Seoul National University, 151-742, Seoul, Korea aut Yi Jongheop School of Chemical and Biological Engineering, Seoul National University, 151-742, Seoul, Korea aut Kim Younghun Department of Chemical Engineering, Kwangwoon University, 139-701, Seoul, Korea aut Korean Journal of Chemical Engineering Springer US; http://www.springer-ny.com 30/3(2013-03-01), 671-674 0256-1115 30:3<671 2013 30 11814 https://doi.org/10.1007/s11814-012-0172-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11814-012-0172-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Roh Jinkyu Department of Chemical Engineering, Kwangwoon University, 139-701, Seoul, Korea aut NATIONALLICENCE 700 1- Umh Ha Department of Chemical Engineering, Kwangwoon University, 139-701, Seoul, Korea aut NATIONALLICENCE 700 1- Sim Jaehoon Department of Chemical Engineering, Kwangwoon University, 139-701, Seoul, Korea aut NATIONALLICENCE 700 1- Park Sumin School of Chemical and Biological Engineering, Seoul National University, 151-742, Seoul, Korea aut NATIONALLICENCE 700 1- Yi Jongheop School of Chemical and Biological Engineering, Seoul National University, 151-742, Seoul, Korea aut NATIONALLICENCE 700 1- Kim Younghun Department of Chemical Engineering, Kwangwoon University, 139-701, Seoul, Korea aut NATIONALLICENCE 773 0- Korean Journal of Chemical Engineering Springer US; http://www.springer-ny.com 30/3(2013-03-01), 671-674 0256-1115 30:3<671 2013 30 11814 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer