naa a22 4500 510820646 CHVBK 20180411083529.0 cr unu---uuuuu 180411e20130401xx s 000 0 eng 10.1007/s11814-012-0202-1 doi (NATIONALLICENCE)springer-10.1007/s11814-012-0202-1 Production of cyclic adenosine-3′,5′-monophosphate by whole cell catalysis using recombinant Escherichia coli overexpressing adenylate cyclase [Elektronische Daten] [Nan Li, Ying He, Yong Chen, Xiaochun Chen, Jianxin Bai, Jinglan Wu, Jingjing Xie, Hanjie Ying] Adenylate cyclase (EC 4.6.1.1) catalyzes the formation of cyclic adenosine-3′,5′-monophosphate (cAMP) from adenosine 5′-triphosphate (ATP). Recombinant Escherichia coli overexpressing adenylate cyclase was used to synthesize cAMP by whole cell catalysis. Some key parameters were examined during the catalytic process, while pH and Mg2+ were found to influence cAMP production significantly. Optimum conditions were pH 8.52 and 30 °C with 77.2 mM Mg2+ in 100 mM Tris-HCl buffer, including 0.25% Triton-X 100 as detergent and 30 mM pyruvate sodium as enzyme activator for 6 h. 14.93 g/L of cAMP was produced with a conversion rate of 91.5%. The current work provided a potential way for the industrial production of cAMP. Korean Institute of Chemical Engineers, Seoul, Korea, 2013 cAMP nationallicence Adenylate Cyclase nationallicence Whole Cell Catalysis nationallicence Bioconversion nationallicence Li Nan State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, 210009, Nanjing, P. R. China aut He Ying State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, 210009, Nanjing, P. R. China aut Chen Yong State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, 210009, Nanjing, P. R. China aut Chen Xiaochun State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, 210009, Nanjing, P. R. China aut Bai Jianxin State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, 210009, Nanjing, P. R. China aut Wu Jinglan State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, 210009, Nanjing, P. R. China aut Xie Jingjing State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, 210009, Nanjing, P. R. China aut Ying Hanjie State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, 210009, Nanjing, P. R. China aut Korean Journal of Chemical Engineering Springer US; http://www.springer-ny.com 30/4(2013-04-01), 913-917 0256-1115 30:4<913 2013 30 11814 https://doi.org/10.1007/s11814-012-0202-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11814-012-0202-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Nan State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, 210009, Nanjing, P. R. China aut NATIONALLICENCE 700 1- He Ying State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, 210009, Nanjing, P. R. China aut NATIONALLICENCE 700 1- Chen Yong State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, 210009, Nanjing, P. R. China aut NATIONALLICENCE 700 1- Chen Xiaochun State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, 210009, Nanjing, P. R. China aut NATIONALLICENCE 700 1- Bai Jianxin State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, 210009, Nanjing, P. R. China aut NATIONALLICENCE 700 1- Wu Jinglan State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, 210009, Nanjing, P. R. China aut NATIONALLICENCE 700 1- Xie Jingjing State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, 210009, Nanjing, P. R. China aut NATIONALLICENCE 700 1- Ying Hanjie State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, 210009, Nanjing, P. R. China aut NATIONALLICENCE 773 0- Korean Journal of Chemical Engineering Springer US; http://www.springer-ny.com 30/4(2013-04-01), 913-917 0256-1115 30:4<913 2013 30 11814 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer