naa a22 4500 510820840 CHVBK 20180411083530.0 cr unu---uuuuu 180411e20130801xx s 000 0 eng 10.1007/s11814-013-0098-4 doi (NATIONALLICENCE)springer-10.1007/s11814-013-0098-4 Highly efficient molecular delivery into Chlamydomonas reinhardtii by electroporation [Elektronische Daten] [Kiyeon Jeon, Arumuganainar Suresh, Yeu-Chun Kim] Electroporation is a highly efficient delivery method for transformation in various cell types; however, in microalgae there is lack of optimized electroporation parameters due to cell wall, protoplast preparation and viability. Therefore, we optimized electroporation conditions for transforming microalgae using Chlamydomonas reinhardtii strains of wild type and mutant (cell wall deficient). To investigate the effects of molecule size, calcein (623 Da) and fluorescein isothiocyanate-dextran (FITC-dextran, 40 kDa) were used and various electroporation parameters were applied such as different voltage and pulse length and molecule uptake pattern and cell viability were observed. Cell wall is insignificant in case of small sized molecule uptake as noticed by 1.25 kV/cm and 30 ms for both strains, whereas for larger molecules by 1.5 and 2 kV/cm and 30 ms for mutant and wild type, respectively. In terms of viability, there was no significant difference in both the strains on applied electroporation parameters. The controlled parameters corresponding to 1.5 to 2.0 kV/cm and 20 to 30 ms could be used to deliver macromolecules (DNA, proteins) into cells effectively. Korean Institute of Chemical Engineers, Seoul, Korea, 2013 Electroporation nationallicence Microalgae nationallicence Chlamydomonas reinhardtii nationallicence Molecules Delivery nationallicence Viability nationallicence Jeon Kiyeon Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), 305-701, Daejeon, Korea aut Suresh Arumuganainar Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), 305-701, Daejeon, Korea aut Kim Yeu-Chun Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), 305-701, Daejeon, Korea aut Korean Journal of Chemical Engineering Springer US; http://www.springer-ny.com 30/8(2013-08-01), 1626-1630 0256-1115 30:8<1626 2013 30 11814 https://doi.org/10.1007/s11814-013-0098-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11814-013-0098-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jeon Kiyeon Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), 305-701, Daejeon, Korea aut NATIONALLICENCE 700 1- Suresh Arumuganainar Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), 305-701, Daejeon, Korea aut NATIONALLICENCE 700 1- Kim Yeu-Chun Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), 305-701, Daejeon, Korea aut NATIONALLICENCE 773 0- Korean Journal of Chemical Engineering Springer US; http://www.springer-ny.com 30/8(2013-08-01), 1626-1630 0256-1115 30:8<1626 2013 30 11814 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer