naa a22 4500 510820875 CHVBK 20180411092759.0 cr unu---uuuuu 180411e20130801xx s 000 0 eng 10.1007/s11814-013-0106-8 doi (NATIONALLICENCE)springer-10.1007/s11814-013-0106-8 A three-zone simulated moving-bed for separation of immunoglobulin Y [Elektronische Daten] [Sung-Moon Song, In Kim] Separation of immunoglobulin Y (IgY) from hen eggs is an interesting topic for investigating antibody purification. Understanding of IgY separation by chromatography is necessary to prepare antibody. IgY preparation in SMB was simulated by Aspen chromatography and was experimented by assembling a 3-zone simulated moving bed (SMB). With change of stream flow rates, the SMB performance was studied. Simulation of IgY batch HPLC chromatography was also done and confirmed by HPLC experiments. Based on these, good operating conditions of SMB chromatography were determined. Three-zone SMB equipment was set up by connecting three C18-HPLC columns, four HPLC pumps, and six multiposition valves. Batch chromatography of IgY was conducted to determine the isotherms of IgY and contaminating impurity. The outlet streams of SMB, raffinate and extract were sampled and analyzed by analytical HPLC system. The adsorption isotherms of IgY and impurity were determined as H IgY =0.1 and H impure =1.1. The highest experimental purity of IgY from SMB was obtained as 98% for the operating parameters of Q feed =0.2mL/min, Q desorbent =0.31 mL/min, Q extract =0.2 mL/min, Q raffinate =0.3 mL/min, and switching time=8.47 min. Korean Institute of Chemical Engineers, Seoul, Korea, 2013 IgY nationallicence SMB (Simulated Moving Bed) nationallicence Song Sung-Moon Department of Chemical Engineering, Chungnam National University, 220, Gung-dong, Yuseong-gu, 305-764, Daejeon, Korea aut Kim In Department of Chemical Engineering, Chungnam National University, 220, Gung-dong, Yuseong-gu, 305-764, Daejeon, Korea aut Korean Journal of Chemical Engineering Springer US; http://www.springer-ny.com 30/8(2013-08-01), 1527-1532 0256-1115 30:8<1527 2013 30 11814 https://doi.org/10.1007/s11814-013-0106-8 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11814-013-0106-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Song Sung-Moon Department of Chemical Engineering, Chungnam National University, 220, Gung-dong, Yuseong-gu, 305-764, Daejeon, Korea aut NATIONALLICENCE 700 1- Kim In Department of Chemical Engineering, Chungnam National University, 220, Gung-dong, Yuseong-gu, 305-764, Daejeon, Korea aut NATIONALLICENCE 773 0- Korean Journal of Chemical Engineering Springer US; http://www.springer-ny.com 30/8(2013-08-01), 1527-1532 0256-1115 30:8<1527 2013 30 11814 Metadata rights reserved Springer special CC-BY-NC licence nationallicence SWISSBIB 494241373 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer