naa a22 4500 510820972 CHVBK 20180411083531.0 cr unu---uuuuu 180411e20130801xx s 000 0 eng 10.1007/s11814-013-0102-z doi (NATIONALLICENCE)springer-10.1007/s11814-013-0102-z Loofah sponge activated by periodate oxidation as a carrier for covalent immobilization of lipase [Elektronische Daten] [Renmin Gong, Jian Zhang, Jun Zhu, Jianting Wang, Qiumei Lai, Bo Jiang] Cellulose in loofah sponge was first oxidized by sodium periodate at positions 2 and 3 of anhydroglucose unit to introduce aldehyde groups, which were able to react with amino groups of lipase to form Schiff's base, and result in loofah sponge immobilized lipase. The result of periodate oxidation was confirmed by determination of aldehyde group content and analysis of Fourier transform infrared spectra. The optimal pH for both free and immobilized lipases was approximately 7.5. The maximum activity of immobilized lipase was observed at 40 °C, while the free lipase exhibited maximum activity at 37 °C. The immobilized lipase presented improved thermal stability, storage stability, and reusability. Korean Institute of Chemical Engineers, Seoul, Korea, 2013 Loofah Sponge nationallicence Periodate Oxidation nationallicence Lipase nationallicence Immobilization nationallicence Gong Renmin State Key Laboratory of Food Science and Technology, Jiangnan University, 214122, Wuxi, P. R. China aut Zhang Jian College of Life Science, Anhui Normal University, 241000, Wuhu, P. R. China aut Zhu Jun College of Life Science, Anhui Normal University, 241000, Wuhu, P. R. China aut Wang Jianting College of Life Science, Anhui Normal University, 241000, Wuhu, P. R. China aut Lai Qiumei College of Life Science, Anhui Normal University, 241000, Wuhu, P. R. China aut Jiang Bo State Key Laboratory of Food Science and Technology, Jiangnan University, 214122, Wuxi, P. R. China aut Korean Journal of Chemical Engineering Springer US; http://www.springer-ny.com 30/8(2013-08-01), 1620-1625 0256-1115 30:8<1620 2013 30 11814 https://doi.org/10.1007/s11814-013-0102-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11814-013-0102-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gong Renmin State Key Laboratory of Food Science and Technology, Jiangnan University, 214122, Wuxi, P. R. China aut NATIONALLICENCE 700 1- Zhang Jian College of Life Science, Anhui Normal University, 241000, Wuhu, P. R. China aut NATIONALLICENCE 700 1- Zhu Jun College of Life Science, Anhui Normal University, 241000, Wuhu, P. R. China aut NATIONALLICENCE 700 1- Wang Jianting College of Life Science, Anhui Normal University, 241000, Wuhu, P. R. China aut NATIONALLICENCE 700 1- Lai Qiumei College of Life Science, Anhui Normal University, 241000, Wuhu, P. R. China aut NATIONALLICENCE 700 1- Jiang Bo State Key Laboratory of Food Science and Technology, Jiangnan University, 214122, Wuxi, P. R. China aut NATIONALLICENCE 773 0- Korean Journal of Chemical Engineering Springer US; http://www.springer-ny.com 30/8(2013-08-01), 1620-1625 0256-1115 30:8<1620 2013 30 11814 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer