naa a22 4500 510821162 CHVBK 20180411083532.0 cr unu---uuuuu 180411e20130501xx s 000 0 eng 10.1007/s11814-012-0219-5 doi (NATIONALLICENCE)springer-10.1007/s11814-012-0219-5 Enhanced production of carboxymethylcellulase by Cellulophaga lytica LBH-14 in pilot-scale bioreactor under optimized conditions involved in dissolved oxygen [Elektronische Daten] [Wa Cao, Sang-Un Lee, Jianhong Li, Jin-Woo Lee] The optimal conditions for the production of carboxymethylcellulase (CMCase) by Cellulophaga lytica LBH-14 at flask scale has been previously reported. In this study, we optimized the parameters involved in dissolved oxygen in 7 and 100 L bioreactors for pilot-scaled production of CMCase by C. lytica LBH-14. The optimal conditions of agitation speed and aeration rate for cell growth in 7 L bioreactors were 395 rpm and 0.98 vvm, whereas those for production of CMCase were 357 rpm and 0.55 vvm. The optimal inner pressures for cell growth and production of CMCase by C. lytica LBH-14 in 100 L bioreactors were 0.00 and 0.06MPa, respectively. The production of CMCase under an optimized inner pressure was 1.38 times higher than that without an inner pressure. The maximal production of CMCase by C. lytica under optimized conditions at pilot scale using rice bran and ammonium chloride was 153.6 U/mL, which was 1.39 times higher than that at flask scale. Korean Institute of Chemical Engineers, Seoul, Korea, 2013 Cellulophaga lytica nationallicence Carboxymethylcellulase nationallicence Marine Microorganism nationallicence Pilot-scaled Production nationallicence Response Surface Method nationallicence Cao Wa Department of Medical Bioscience, Graduate School of Dong-A University, 604-714, Busan, Korea aut Lee Sang-Un Department of Medical Bioscience, Graduate School of Dong-A University, 604-714, Busan, Korea aut Li Jianhong College of Plant Science & Technology, Huazhong Agricultural University, 430070, Wuhan, Hebei, China aut Lee Jin-Woo BK21 Bio-Silver Program of Dong-A, 604-714, Busan, Korea aut Korean Journal of Chemical Engineering Springer US; http://www.springer-ny.com 30/5(2013-05-01), 1105-1110 0256-1115 30:5<1105 2013 30 11814 https://doi.org/10.1007/s11814-012-0219-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11814-012-0219-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cao Wa Department of Medical Bioscience, Graduate School of Dong-A University, 604-714, Busan, Korea aut NATIONALLICENCE 700 1- Lee Sang-Un Department of Medical Bioscience, Graduate School of Dong-A University, 604-714, Busan, Korea aut NATIONALLICENCE 700 1- Li Jianhong College of Plant Science & Technology, Huazhong Agricultural University, 430070, Wuhan, Hebei, China aut NATIONALLICENCE 700 1- Lee Jin-Woo BK21 Bio-Silver Program of Dong-A, 604-714, Busan, Korea aut NATIONALLICENCE 773 0- Korean Journal of Chemical Engineering Springer US; http://www.springer-ny.com 30/5(2013-05-01), 1105-1110 0256-1115 30:5<1105 2013 30 11814 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer