naa a22 4500 510821189 CHVBK 20180411083533.0 cr unu---uuuuu 180411e20130501xx s 000 0 eng 10.1007/s11814-013-0010-2 doi (NATIONALLICENCE)springer-10.1007/s11814-013-0010-2 Simulation of computational fluid dynamics and comparison of cephalosporin C fermentation performance with different impeller combinations [Elektronische Daten] [Shengbing Duan, Guoqiang Yuan, Yanli Zhao, Weijia Ni, Hongzhen Luo, Zhongping Shi, Fan Liu] Cephalosporin C (CPC) fermentation by Acremonium chrysogenum is an extremely high oxygen-consuming process and oxygen transfer rate in a bioreactor directly affects fermentation performance. In this study, fluid dynamics and oxygen transfer in a 7 L bioreactor with different impellers combinations were simulated by computational fluid dynamics (CFD) model. Based on the simulation results, two impeller combinations with higher oxygen transfer rate (K L a) were selected to conduct CPC fermentations, aiming at achieving high CPC concentration and low accumulation of major by-product, deacetoxycephalosporin (DAOC). It was found that an impeller combination with a higher K L a and moderate shear force is the prerequisite for efficient CPC production in a stirred bioreactor. The best impeller combination, which installed a six-bladed turbine and a four-pitched-blade turbine at bottom and upper layers but with a shortened impellers inter-distance, produced the highest CPC concentration of 35.77 g/L and lowest DAOC/CPC ratio of 0.5%. Korean Institute of Chemical Engineers, Seoul, Korea, 2013 A. chrysogenum nationallicence Antibiotic nationallicence Cephalosporin C Production nationallicence CFD nationallicence Shear Force nationallicence Duan Shengbing Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 214122, Wuxi, Jiangsu, China aut Yuan Guoqiang CSPC Hebei Zhongrun Pharmaceutical Co., Ltd., 050041, Shijiazhuang, Hebei, China aut Zhao Yanli CSPC Hebei Zhongrun Pharmaceutical Co., Ltd., 050041, Shijiazhuang, Hebei, China aut Ni Weijia Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 214122, Wuxi, Jiangsu, China aut Luo Hongzhen Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 214122, Wuxi, Jiangsu, China aut Shi Zhongping Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 214122, Wuxi, Jiangsu, China aut Liu Fan Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 214122, Wuxi, Jiangsu, China aut Korean Journal of Chemical Engineering Springer US; http://www.springer-ny.com 30/5(2013-05-01), 1097-1104 0256-1115 30:5<1097 2013 30 11814 https://doi.org/10.1007/s11814-013-0010-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11814-013-0010-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Duan Shengbing Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 214122, Wuxi, Jiangsu, China aut NATIONALLICENCE 700 1- Yuan Guoqiang CSPC Hebei Zhongrun Pharmaceutical Co., Ltd., 050041, Shijiazhuang, Hebei, China aut NATIONALLICENCE 700 1- Zhao Yanli CSPC Hebei Zhongrun Pharmaceutical Co., Ltd., 050041, Shijiazhuang, Hebei, China aut NATIONALLICENCE 700 1- Ni Weijia Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 214122, Wuxi, Jiangsu, China aut NATIONALLICENCE 700 1- Luo Hongzhen Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 214122, Wuxi, Jiangsu, China aut NATIONALLICENCE 700 1- Shi Zhongping Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 214122, Wuxi, Jiangsu, China aut NATIONALLICENCE 700 1- Liu Fan Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 214122, Wuxi, Jiangsu, China aut NATIONALLICENCE 773 0- Korean Journal of Chemical Engineering Springer US; http://www.springer-ny.com 30/5(2013-05-01), 1097-1104 0256-1115 30:5<1097 2013 30 11814 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer