naa a22 4500 510821448 CHVBK 20180411083534.0 cr unu---uuuuu 180411e20130201xx s 000 0 eng 10.1007/s11814-012-0134-9 doi (NATIONALLICENCE)springer-10.1007/s11814-012-0134-9 A new flow path design for multidimensional protein identification technology using nano-liquid chromatography electrospray ionization mass spectrometry [Elektronische Daten] [Thangamani Rajesh, Hyung-Yeon Park, Eunjung Song, Changmin Sung, Sung-Hee Park, Jae-Hun Lee, Dongwon Yoo, Yun-Gon Kim, Jong-Min Jeon, Byung-Gee Kim, Yung-Hun Yang] Multidimensional protein identification technology (MudPIT) is one of the most versatile methods for separating and identifying highly complex peptides or proteins. However, there are still inherent problems resulting from salt in eluent systems and instrumentation with MudPIT. We designed a novel and simple flow path using two-valve system and successfully performed a fully automated peptide analysis using MudPIT coupled with nano-liquid chromatography electrospray ionization mass spectrometry (nLC-ESI-MS). It enables to generate a remarkably stable nanospray during the MudPIT analysis and realize the fully automated MudPIT system. This column arrangement could be easily installed to avoid laborious loading steps and unstable ionization from discontinuous flow. Consequently, the new flow path design for MudPIT system guarantees the detection of more peptides and higher protein coverage in proteome analysis. Korean Institute of Chemical Engineers, Seoul, Korea, 2012 MudPIT nationallicence Nano LC-ESI-MS nationallicence SCX nationallicence C18 nationallicence Peptide Identification nationallicence Rajesh Thangamani Department of Microbial Engineering, College of Engineering, Konkuk University, 1, Hwayang-dong, Gwangjin-gu, 143-701, Seoul, Korea aut Park Hyung-Yeon Bio-MAX Institute, Seoul National University, Gwanak-gu, 151-742, Seoul, Korea aut Song Eunjung School of Chemical and Biological Engineering, Institute of Molecular Biology and Genetics, Seoul National University, Gwanak-gu, 151-742, Seoul, Korea aut Sung Changmin School of Chemical and Biological Engineering, Institute of Molecular Biology and Genetics, Seoul National University, Gwanak-gu, 151-742, Seoul, Korea aut Park Sung-Hee School of Chemical and Biological Engineering, Institute of Molecular Biology and Genetics, Seoul National University, Gwanak-gu, 151-742, Seoul, Korea aut Lee Jae-Hun School of Chemical and Biological Engineering, Institute of Molecular Biology and Genetics, Seoul National University, Gwanak-gu, 151-742, Seoul, Korea aut Yoo Dongwon School of Chemical and Biological Engineering, Institute of Molecular Biology and Genetics, Seoul National University, Gwanak-gu, 151-742, Seoul, Korea aut Kim Yun-Gon Chemical Engineering, Soongsil University, 511, Sangdo-dong, 156-743, Seoul, Korea aut Jeon Jong-Min Department of Microbial Engineering, College of Engineering, Konkuk University, 1, Hwayang-dong, Gwangjin-gu, 143-701, Seoul, Korea aut Kim Byung-Gee School of Chemical and Biological Engineering, Institute of Molecular Biology and Genetics, Seoul National University, Gwanak-gu, 151-742, Seoul, Korea aut Yang Yung-Hun Department of Microbial Engineering, College of Engineering, Konkuk University, 1, Hwayang-dong, Gwangjin-gu, 143-701, Seoul, Korea aut Korean Journal of Chemical Engineering Springer US; http://www.springer-ny.com 30/2(2013-02-01), 417-421 0256-1115 30:2<417 2013 30 11814 https://doi.org/10.1007/s11814-012-0134-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11814-012-0134-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rajesh Thangamani Department of Microbial Engineering, College of Engineering, Konkuk University, 1, Hwayang-dong, Gwangjin-gu, 143-701, Seoul, Korea aut NATIONALLICENCE 700 1- Park Hyung-Yeon Bio-MAX Institute, Seoul National University, Gwanak-gu, 151-742, Seoul, Korea aut NATIONALLICENCE 700 1- Song Eunjung School of Chemical and Biological Engineering, Institute of Molecular Biology and Genetics, Seoul National University, Gwanak-gu, 151-742, Seoul, Korea aut NATIONALLICENCE 700 1- Sung Changmin School of Chemical and Biological Engineering, Institute of Molecular Biology and Genetics, Seoul National University, Gwanak-gu, 151-742, Seoul, Korea aut NATIONALLICENCE 700 1- Park Sung-Hee School of Chemical and Biological Engineering, Institute of Molecular Biology and Genetics, Seoul National University, Gwanak-gu, 151-742, Seoul, Korea aut NATIONALLICENCE 700 1- Lee Jae-Hun School of Chemical and Biological Engineering, Institute of Molecular Biology and Genetics, Seoul National University, Gwanak-gu, 151-742, Seoul, Korea aut NATIONALLICENCE 700 1- Yoo Dongwon School of Chemical and Biological Engineering, Institute of Molecular Biology and Genetics, Seoul National University, Gwanak-gu, 151-742, Seoul, Korea aut NATIONALLICENCE 700 1- Kim Yun-Gon Chemical Engineering, Soongsil University, 511, Sangdo-dong, 156-743, Seoul, Korea aut NATIONALLICENCE 700 1- Jeon Jong-Min Department of Microbial Engineering, College of Engineering, Konkuk University, 1, Hwayang-dong, Gwangjin-gu, 143-701, Seoul, Korea aut NATIONALLICENCE 700 1- Kim Byung-Gee School of Chemical and Biological Engineering, Institute of Molecular Biology and Genetics, Seoul National University, Gwanak-gu, 151-742, Seoul, Korea aut NATIONALLICENCE 700 1- Yang Yung-Hun Department of Microbial Engineering, College of Engineering, Konkuk University, 1, Hwayang-dong, Gwangjin-gu, 143-701, Seoul, Korea aut NATIONALLICENCE 773 0- Korean Journal of Chemical Engineering Springer US; http://www.springer-ny.com 30/2(2013-02-01), 417-421 0256-1115 30:2<417 2013 30 11814 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer