naa a22 4500 510821464 CHVBK 20180411083534.0 cr unu---uuuuu 180411e20130201xx s 000 0 eng 10.1007/s11814-012-0132-y doi (NATIONALLICENCE)springer-10.1007/s11814-012-0132-y Lipase from Penicillium camembertii KCCM 11268: Optimization of solid state fermentation and application to biodiesel production [Elektronische Daten] [Waraporn Malilas, Seong Kang, Sung Kim, Hah Yoo, Warawut Chulalaksananukul, Seung Kim] Lipase was produced by Penicillium camembertii KCCM 11268 under solid state fermentation (SSF), and the production process was optimized by using statistical experimental designs. The initial moisture content, cultivation time, inoculum size and concentration of basal medium were considered as the factors of optimum conditions for SSF. P. Camembertii KCCM 11268 was cultivated in SSF using wheat bran as the substrate for lipase production. Under the optimized condition, lipase activity was reached around 7.8 U/ml after eight days fermentation. To partially purify the lipase, ammonium sulfate (80% saturation) was added to the crude lipase solution and concentrated using a diafiltration (VIVAFLOW 50). The concentrated lipase solution from P. Camembertii KCCM 11268 (PCL) was immobilized on silica gel by cross-linking method. Also, PCL was mixed with a commercial lipase solution from Candida rugosa (CRL), and this mixture was co-immobilized on silica gel. The immobilized and co-immobilized lipase activities were 1150.1 and 7924.8 U/g matrix, respectively. Palm oil and methanol were used as substrates and 1mmol of methanol was added every 1.5 h and 2 times during biodiesel production. The reaction was carried out at temperatures of 30, 40, 50, 60 and 70 °C. The maximum biodiesel conversion by co-immobilized lipase was 99% after 5 h at 50 °C. Korean Institute of Chemical Engineers, Seoul, Korea, 2012 Penicillium camembertii KCCM 11268 nationallicence Solid State Fermentation nationallicence Lipase Production nationallicence Statistical Experimental Design nationallicence Biodiesel Production nationallicence Immobilization nationallicence Malilas Waraporn Department of Chemical and Biological Engineering, Korea University, 1, Anam-dong, Sungbuk-gu, 136-701, Seoul, Korea aut Kang Seong Department of Chemical and Biological Engineering, Korea University, 1, Anam-dong, Sungbuk-gu, 136-701, Seoul, Korea aut Kim Sung Department of Chemical and Biological Engineering, Korea University, 1, Anam-dong, Sungbuk-gu, 136-701, Seoul, Korea aut Yoo Hah Department of Chemical and Biological Engineering, Korea University, 1, Anam-dong, Sungbuk-gu, 136-701, Seoul, Korea aut Chulalaksananukul Warawut Department of Botany, Faculty of Science, Chulalongkorn University, Bangkok, Thailand aut Kim Seung Department of Chemical and Biological Engineering, Korea University, 1, Anam-dong, Sungbuk-gu, 136-701, Seoul, Korea aut Korean Journal of Chemical Engineering Springer US; http://www.springer-ny.com 30/2(2013-02-01), 405-412 0256-1115 30:2<405 2013 30 11814 https://doi.org/10.1007/s11814-012-0132-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11814-012-0132-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Malilas Waraporn Department of Chemical and Biological Engineering, Korea University, 1, Anam-dong, Sungbuk-gu, 136-701, Seoul, Korea aut NATIONALLICENCE 700 1- Kang Seong Department of Chemical and Biological Engineering, Korea University, 1, Anam-dong, Sungbuk-gu, 136-701, Seoul, Korea aut NATIONALLICENCE 700 1- Kim Sung Department of Chemical and Biological Engineering, Korea University, 1, Anam-dong, Sungbuk-gu, 136-701, Seoul, Korea aut NATIONALLICENCE 700 1- Yoo Hah Department of Chemical and Biological Engineering, Korea University, 1, Anam-dong, Sungbuk-gu, 136-701, Seoul, Korea aut NATIONALLICENCE 700 1- Chulalaksananukul Warawut Department of Botany, Faculty of Science, Chulalongkorn University, Bangkok, Thailand aut NATIONALLICENCE 700 1- Kim Seung Department of Chemical and Biological Engineering, Korea University, 1, Anam-dong, Sungbuk-gu, 136-701, Seoul, Korea aut NATIONALLICENCE 773 0- Korean Journal of Chemical Engineering Springer US; http://www.springer-ny.com 30/2(2013-02-01), 405-412 0256-1115 30:2<405 2013 30 11814 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer