naa a22 4500 510823629 CHVBK 20180411083545.0 cr unu---uuuuu 180411e20130401xx s 000 0 eng 10.1007/s12541-013-0091-1 doi (NATIONALLICENCE)springer-10.1007/s12541-013-0091-1 Investigation of various ac electrokinetics in a microniche system for cell separation [Elektronische Daten] [Minchurl Kim, Youngwoong Kim, Byungkyu Kim] We investigate various AC electrokinetic (dielectrophoresis, electro-thermal and electro-osmosis) phenomena in a micro-niche system for negative-dielectrophoresis (n-dep) based cell separation. For that purpose, an experimental system is constructed. The system is comprised of a slit-type electrode array to apply voltage, a micro cam-scope to monitor cell movement, micro stages to adjust the focal length of the cam-scope properly, a function generator to apply voltage with various frequencies, and an illumination system. After the conductivity of the medium and the magnitude and frequency of the voltage are selected as experiment variables, cell movement is investigated. The ranges of each variable studied are 0.001 ∼ 0.2 S/m, 1 ∼ 7 Vpp, and 100 Hz ∼ 1 MHz respectively. For the target cells, k562 cells of a leukemia cell line were employed. In the experimental study, n-dep is most dominant over other electrokinetic phenomena with 5 Vpp of input voltage and in the range of 100 ∼ 500 kHz although the theoretical equation indicates that the higher voltage (7 Vpp) generates a higher n-dep force. This is because the higher voltage causes not only a higher n-dep force but also higher electro-thermal and electro-osmosis. Based on the obtained experimental results, we expect to establish a reliable n-dep force based cell sorting system in the near future. Korean Society for Precision Engineering and Springer-Verlag Berlin Heidelberg, 2013 AC electrokinetics nationallicence Micro-niche system nationallicence Cell separation nationallicence Negative dielectrophoretic force nationallicence Electro osmosis nationallicence Electro thermal nationallicence Kim Minchurl School of Aerospace and Mechanical Engineering, Korea Aerospace University, 412-791, Goyang-si, Gyeonggi-do, Korea aut Kim Youngwoong School of Aerospace and Mechanical Engineering, Korea Aerospace University, 412-791, Goyang-si, Gyeonggi-do, Korea aut Kim Byungkyu School of Aerospace and Mechanical Engineering, Korea Aerospace University, 412-791, Goyang-si, Gyeonggi-do, Korea aut International Journal of Precision Engineering and Manufacturing Korean Society for Precision Engineering 14/4(2013-04-01), 675-678 2234-7593 14:4<675 2013 14 12541 https://doi.org/10.1007/s12541-013-0091-1 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12541-013-0091-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kim Minchurl School of Aerospace and Mechanical Engineering, Korea Aerospace University, 412-791, Goyang-si, Gyeonggi-do, Korea aut NATIONALLICENCE 700 1- Kim Youngwoong School of Aerospace and Mechanical Engineering, Korea Aerospace University, 412-791, Goyang-si, Gyeonggi-do, Korea aut NATIONALLICENCE 700 1- Kim Byungkyu School of Aerospace and Mechanical Engineering, Korea Aerospace University, 412-791, Goyang-si, Gyeonggi-do, Korea aut NATIONALLICENCE 773 0- International Journal of Precision Engineering and Manufacturing Korean Society for Precision Engineering 14/4(2013-04-01), 675-678 2234-7593 14:4<675 2013 14 12541 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer