naa a22 4500 528782339 20180924065513.0 cr unu---uuuuu 180924e20120227xx s 000 0 eng 10.3929/ethz-b-000048456 doi 10.1371/journal.pone.0032724 doi (ETHRESEARCH)oai:www.research-collection.ethz.ch:20.500.11850/48456 Deep-Sequencing Protocols Influence the Results Obtained in Small-RNA Sequencing [Elektronische Daten] [Joern Toedling, Nicolas Servant, Constance Ciaudo, Laurent Farinelli, Olivier Voinnet, Edith Heard, Emmanuel Barillot] PLoS ONE Open access ethresearch Second-generation sequencing is a powerful method for identifying and quantifying small-RNA components of cells. However, little attention has been paid to the effects of the choice of sequencing platform and library preparation protocol on the results obtained. We present a thorough comparison of small-RNA sequencing libraries generated from the same embryonic stem cell lines, using different sequencing platforms, which represent the three major second-generation sequencing technologies, and protocols. We have analysed and compared the expression of microRNAs, as well as populations of small RNAs derived from repetitive elements. Despite the fact that different libraries display a good correlation between sequencing platforms, qualitative and quantitative variations in the results were found, depending on the protocol used. Thus, when comparing libraries from different biological samples, it is strongly recommended to use the same sequencing platform and protocol in order to ensure the biological relevance of the comparisons. Creative Commons Attribution 3.0 Unported http://creativecommons.org/licenses/by/3.0 ethresearch Toedling Joern joint author Servant Nicolas joint author Ciaudo Constance joint author Farinelli Laurent joint author Voinnet Olivier joint author Heard Edith joint author Barillot Emmanuel joint author PLoS ONE S.l. : Public Library of Science 7 (2), p. e32724 1932-6203 http://hdl.handle.net/20.500.11850/48456 text/html WWW-Backlink auf das Repository (Open access) 1 Journal Article ethresearch ETHRESEARCH 856 40 http://hdl.handle.net/20.500.11850/48456 text/html WWW-Backlink auf das Repository (Open access) ETHRESEARCH 700 1- Toedling Joern joint author ETHRESEARCH 700 1- Servant Nicolas joint author ETHRESEARCH 700 1- Ciaudo Constance joint author ETHRESEARCH 700 1- Farinelli Laurent joint author ETHRESEARCH 700 1- Voinnet Olivier joint author ETHRESEARCH 700 1- Heard Edith joint author ETHRESEARCH 700 1- Barillot Emmanuel joint author ETHRESEARCH 773 0- PLoS ONE S.l. : Public Library of Science 7 (2), p. e32724 1932-6203 BK010053 XK010053 XK010000 ETHRESEARCH ETHRESEARCH ETHRESEARCH Journal Article Open access