naa a22 4500 528782401 20180924065513.0 cr unu---uuuuu 180924e20121008xx s 000 0 eng 10.3929/ethz-b-000082034 doi 10.1371/journal.pone.0046755 doi (ETHRESEARCH)oai:www.research-collection.ethz.ch:20.500.11850/82034 Uterine NK cells are critical in shaping DC immunogenic functions compatible with pregnancy progression [Elektronische Daten] [Irene T. Gonzalez, Gabriela Barrientos, Nancy Freitag, Teresa Otto, Victor L. Thijssen, Petra Moschansky, Petra von Kwiatkowski, Burghard F. Klapp, Elke Winterhager, Stefan Bauersachs, Sandra M. Blois] PLoS ONE Open access ethresearch Dendritic cell (DC) and natural killer (NK) cell interactions are important for the regulation of innate and adaptive immunity, but their relevance during early pregnancy remains elusive. Using two different strategies to manipulate the frequency of NK cells and DC during gestation, we investigated their relative impact on the decidualization process and on angiogenic responses that characterize murine implantation. Manipulation of the frequency of NK cells, DC or both lead to a defective decidual response characterized by decreased proliferation and differentiation of stromal cells. Whereas no detrimental effects were evident upon expansion of DC, NK cell ablation in such expanded DC mice severely compromised decidual development and led to early pregnancy loss. Pregnancy failure in these mice was associated with an unbalanced production of anti-angiogenic signals and most notably, with increased expression of genes related to inflammation and immunogenic activation of DC. Thus, NK cells appear to play an important role counteracting potential anomalies raised by DC expansion and overactivity in the decidua, becoming critical for normal pregnancy progression. Creative Commons Attribution 3.0 Unported http://creativecommons.org/licenses/by/3.0 ethresearch Gonzalez Irene T. joint author Barrientos Gabriela joint author Freitag Nancy joint author Otto Teresa joint author Thijssen Victor L. joint author Moschansky Petra joint author Kwiatkowski Petra von joint author Klapp Burghard F. joint author Winterhager Elke joint author Bauersachs Stefan joint author Blois Sandra M. joint author PLoS ONE San Francisco, CA : Public Library of Science 7 (10), p. e46755 1932-6203 http://hdl.handle.net/20.500.11850/82034 text/html WWW-Backlink auf das Repository (Open access) 1 Journal Article ethresearch ETHRESEARCH 856 40 http://hdl.handle.net/20.500.11850/82034 text/html WWW-Backlink auf das Repository (Open access) ETHRESEARCH 700 1- Gonzalez Irene T. joint author ETHRESEARCH 700 1- Barrientos Gabriela joint author ETHRESEARCH 700 1- Freitag Nancy joint author ETHRESEARCH 700 1- Otto Teresa joint author ETHRESEARCH 700 1- Thijssen Victor L. joint author ETHRESEARCH 700 1- Moschansky Petra joint author ETHRESEARCH 700 1- Kwiatkowski Petra von joint author ETHRESEARCH 700 1- Klapp Burghard F. joint author ETHRESEARCH 700 1- Winterhager Elke joint author ETHRESEARCH 700 1- Bauersachs Stefan joint author ETHRESEARCH 700 1- Blois Sandra M. joint author ETHRESEARCH 773 0- PLoS ONE San Francisco, CA : Public Library of Science 7 (10), p. e46755 1932-6203 BK010053 XK010053 XK010000 ETHRESEARCH ETHRESEARCH ETHRESEARCH Journal Article Open access