naa a22 4500 528782541 20180924065513.0 cr unu---uuuuu 180924e20130114xx s 000 0 eng 10.3929/ethz-b-000064241 doi 10.1371/journal.pone.0054196 doi (ETHRESEARCH)oai:www.research-collection.ethz.ch:20.500.11850/64241 Comparative RNA-Seq Analysis of Early-Infected Peach Leaves by the Invasive Phytopathogen Xanthomonas arboricola pv. pruni [Elektronische Daten] [Didier Socquet-Juglard, Tim Kamber, Joël F. Pothier, Danilo Christen, Cesare Gessler, Brion Duffy, Andrea Patocchi] PLoS ONE Open access ethresearch Xanthomonas arboricola pv. pruni is a quarantine bacterial pathogen that threatens peach production by causing necrotic spots on leaves and fruits, thus with the potential of severely reducing yields. The current understanding of the host plant defense responses to the pathogen is very limited. Using whole transcriptome sequencing, differential gene expression was analyzed at two time points, 2 h and 12 h post inoculation (hpi), by comparing the inoculated samples to their respective controls. On the total of 19,781 known peach genes that were expressed in all time points and conditions, 34 and 263 were differentially expressed at 2 and 12 hpi, respectively. Of those, 82% and 40% were up-regulated, respectively; and 18% and 60% were down-regulated, respectively. The functional annotation based on gene ontology (GO) analysis highlighted that genes involved in metabolic process and response to stress were particularly represented at 2 hpi whereas at 12 hpi cellular and metabolic processes were the categories with the highest number of genes differentially expressed. Of particular interest among the differentially expressed genes identified were several pathogen-associated molecular pattern (PAMP) receptors, disease resistance genes including several RPM1-like and pathogenesis related thaumatin encoding genes. Other genes involved in photosynthesis, in cell wall reorganization, in hormone signaling pathways or encoding cytochrome were also differentially expressed. In addition, novel transcripts were identified, providing another basis for further characterization of plant defense-related genes. Overall, this study gives a first insight of the peach defense mechanisms during the very early stages of infection with a bacterial disease in the case of a compatible interaction. Creative Commons Attribution 3.0 Unported http://creativecommons.org/licenses/by/3.0 ethresearch Socquet-Juglard Didier joint author Kamber Tim joint author Pothier Joël F. joint author Christen Danilo joint author Gessler Cesare joint author Duffy Brion joint author Patocchi Andrea joint author PLoS ONE San Francisco, CA, USA : Public Library Science 8 (1), p. e54196 1932-6203 http://hdl.handle.net/20.500.11850/64241 text/html WWW-Backlink auf das Repository (Open access) 1 Journal Article ethresearch ETHRESEARCH 856 40 http://hdl.handle.net/20.500.11850/64241 text/html WWW-Backlink auf das Repository (Open access) ETHRESEARCH 700 1- Socquet-Juglard Didier joint author ETHRESEARCH 700 1- Kamber Tim joint author ETHRESEARCH 700 1- Pothier Joël F. joint author ETHRESEARCH 700 1- Christen Danilo joint author ETHRESEARCH 700 1- Gessler Cesare joint author ETHRESEARCH 700 1- Duffy Brion joint author ETHRESEARCH 700 1- Patocchi Andrea joint author ETHRESEARCH 773 0- PLoS ONE San Francisco, CA, USA : Public Library Science 8 (1), p. e54196 1932-6203 BK010053 XK010053 XK010000 ETHRESEARCH ETHRESEARCH ETHRESEARCH Journal Article Open access