naa a22 4500 528782576 20180924065513.0 cr unu---uuuuu 180924e20120703xx s 000 0 eng 10.3929/ethz-b-000052174 doi 10.1371/journal.pone.0039194 doi (ETHRESEARCH)oai:www.research-collection.ethz.ch:20.500.11850/52174 Otero-Muras Irene Characterizing Multistationarity Regimes in Biochemical Reaction Networks [Elektronische Daten] [Irene Otero-Muras, Julio R. Banga, Antonio A. Alonso] PLoS ONE Open access ethresearch Switch like responses appear as common strategies in the regulation of cellular systems. Here we present a method to characterize bistable regimes in biochemical reaction networks that can be of use to both direct and reverse engineering of biological switches. In the design of a synthetic biological switch, it is important to study the capability for bistability of the underlying biochemical network structure. Chemical Reaction Network Theory (CRNT) may help at this level to decide whether a given network has the capacity for multiple positive equilibria, based on their structural properties. However, in order to build a working switch, we also need to ensure that the bistability property is robust, by studying the conditions leading to the existence of two different steady states. In the reverse engineering of biological switches, knowledge collected about the bistable regimes of the underlying potential model structures can contribute at the model identification stage to a drastic reduction of the feasible region in the parameter space of search. In this work, we make use and extend previous results of the CRNT, aiming not only to discriminate whether a biochemical reaction network can exhibit multiple steady states, but also to determine the regions within the whole space of parameters capable of producing multistationarity. To that purpose we present and justify a condition on the parameters of biochemical networks for the appearance of multistationarity, and propose an efficient and reliable computational method to check its satisfaction through the parameter space. Creative Commons Attribution 3.0 Unported http://creativecommons.org/licenses/by/3.0 ethresearch Banga Julio R. joint author Alonso Antonio A. joint author PLoS ONE S.l. : Public Library of Science 7 (7), p. e39194 1932-6203 http://hdl.handle.net/20.500.11850/52174 text/html WWW-Backlink auf das Repository (Open access) 1 Journal Article ethresearch ETHRESEARCH 856 40 http://hdl.handle.net/20.500.11850/52174 text/html WWW-Backlink auf das Repository (Open access) ETHRESEARCH 100 1- Otero-Muras Irene ETHRESEARCH 700 1- Banga Julio R. joint author ETHRESEARCH 700 1- Alonso Antonio A. joint author ETHRESEARCH 773 0- PLoS ONE S.l. : Public Library of Science 7 (7), p. e39194 1932-6203 BK010053 XK010053 XK010000 ETHRESEARCH ETHRESEARCH ETHRESEARCH Journal Article Open access