β1 integrin-mediated adhesion signalling is essential for epidermal progenitor cell expansion
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| 024 | 7 | 0 | |a 10.3929/ethz-b-000015785 |2 doi |
| 024 | 7 | 0 | |a 10.1371/journal.pone.0005488 |2 doi |
| 035 | |a (ETHRESEARCH)oai:www.research-collecti.ethz.ch:20.500.11850/15785 | ||
| 245 | 0 | 0 | |a β1 integrin-mediated adhesion signalling is essential for epidermal progenitor cell expansion |h [Elektronische Daten] |c [Aleksandra Piwko-Czuchra, Heidi Koegel, Hannelore Meyer, Martina Bauer, Sabine Werner, Cord Brakebusch, Reinhard Fässler] |
| 246 | 0 | |a PLoS ONE | |
| 506 | |a Open access |2 ethresearch | ||
| 520 | 3 | |a Background There is a major discrepancy between the in vitro and in vivo results regarding the role of β1 integrins in the maintenance of epidermal stem/progenitor cells. Studies of mice with skin-specific ablation of β1 integrins suggested that epidermis can form and be maintained in their absence, while in vitro data have shown a fundamental role for these adhesion receptors in stem/progenitor cell expansion and differentiation. Methodology/Principal Findings To elucidate this discrepancy we generated hypomorphic mice expressing reduced β1 integrin levels on keratinocytes that developed similar, but less severe defects than mice with β1-deficient keratinocytes. Surprisingly we found that upon aging these abnormalities attenuated due to a rapid expansion of cells, which escaped or compensated for the down-regulation of β1 integrin expression. A similar phenomenon was observed in aged mice with a complete, skin-specific ablation of the β1 integrin gene, where cells that escaped Cre-mediated recombination repopulated the mutant skin in a very short time period. The expansion of β1 integrin expressing keratinocytes was even further accelerated in situations of increased keratinocyte proliferation such as wound healing. Conclusions/Significance These data demonstrate that expression of β1 integrins is critically important for the expansion of epidermal progenitor cells to maintain epidermal homeostasis. | |
| 540 | |a Creative Commons Attribution 3.0 Unported |u http://creativecommons.org/licenses/by/3.0 |2 ethresearch | ||
| 700 | 1 | |a Piwko-Czuchra |D Aleksandra |e joint author | |
| 700 | 1 | |a Koegel |D Heidi |e joint author | |
| 700 | 1 | |a Meyer |D Hannelore |e joint author | |
| 700 | 1 | |a Bauer |D Martina |e joint author | |
| 700 | 1 | |a Werner |D Sabine |e joint author | |
| 700 | 1 | |a Brakebusch |D Cord |e joint author | |
| 700 | 1 | |a Fässler |D Reinhard |e joint author | |
| 773 | 0 | |t PLoS ONE |d San Francisco, CA : Public Library of Science |g 4 (5), p. e5488 |x 1932-6203 | |
| 856 | 4 | 0 | |u http://hdl.handle.net/20.500.11850/15785 |q text/html |z WWW-Backlink auf das Repository (Open access) |
| 908 | |D 1 |a Journal Article |2 ethresearch | ||
| 950 | |B ETHRESEARCH |P 856 |E 40 |u http://hdl.handle.net/20.500.11850/15785 |q text/html |z WWW-Backlink auf das Repository (Open access) | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Piwko-Czuchra |D Aleksandra |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Koegel |D Heidi |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Meyer |D Hannelore |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Bauer |D Martina |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Werner |D Sabine |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Brakebusch |D Cord |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Fässler |D Reinhard |e joint author | ||
| 950 | |B ETHRESEARCH |P 773 |E 0- |t PLoS ONE |d San Francisco, CA : Public Library of Science |g 4 (5), p. e5488 |x 1932-6203 | ||
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 949 | |B ETHRESEARCH |F ETHRESEARCH |b ETHRESEARCH |j Journal Article |c Open access | ||