caa a22 4500 528783475 20181103072611.0 cr unu---uuuuu 180924e20140320xx s 000 0 eng 10.3929/ethz-b-000082833 doi 10.1371/journal.pone.0092474 doi (ETHRESEARCH)oai:www.research-collection.ethz.ch:20.500.11850/82833 Differences between the Cell Populations from the Peritenon and the Tendon Core with Regard to Their Potential Implication in Tendon Repair [Elektronische Daten] [Jess G. Snedeker, Jennifer A. Cadby, Evelyne Buehler, Charles Godbout, P. Rene van Weeren] PLoS ONE Open access ethresearch The role of intrinsic and extrinsic healing in injured tendons is still debated. In this study, we characterized cell plasticity, proliferative capacity, and migration characteristics as proxy measures of healing potential in cells derived from the peritenon (extrinsic healing) and compared these to cells from the tendon core (intrinsic healing). Both cell populations were extracted from horse superficial digital flexor tendon and characterized for tenogenic and matrix remodeling markers as well as for rates of migration and replication. Furthermore, colony-forming unit assays, multipotency assays, and real-time quantitative polymerase chain reaction analyses of markers of osteogenic and adipogenic differentiation after culture in induction media were performed. Finally, cellular capacity for differentiation towards a myofibroblastic phenotype was assessed. Our results demonstrate that both tendon- and peritenon-derived cell populations are capable of adipogenic and osteogenic differentiation, with higher expression of progenitor cell markers in peritenon cells. Cells from the peritenon also migrated faster, replicate more quickly, and show higher differentiation potential toward a myofibroblastic phenotype when compared to cells from the tendon core. Based on these data, we suggest that cells from the peritenon have substantial potential to influence tendon-healing outcome, warranting further scrutiny of their role. Creative Commons Attribution 4.0 International http://creativecommons.org/licenses/by/4.0 ethresearch Snedeker Jess G. joint author Cadby Jennifer A. joint author Buehler Evelyne joint author Godbout Charles joint author van Weeren P. Rene joint author PLoS ONE Lawrence, Kan : Public Library of Science 9 (3), p. 92474 1932-6203 http://hdl.handle.net/20.500.11850/82833 text/html WWW-Backlink auf das Repository (Open access) 1 Journal Article ethresearch ETHRESEARCH 856 40 http://hdl.handle.net/20.500.11850/82833 text/html WWW-Backlink auf das Repository (Open access) ETHRESEARCH 700 1- Snedeker Jess G. joint author ETHRESEARCH 700 1- Cadby Jennifer A. joint author ETHRESEARCH 700 1- Buehler Evelyne joint author ETHRESEARCH 700 1- Godbout Charles joint author ETHRESEARCH 700 1- van Weeren P. Rene joint author ETHRESEARCH 773 0- PLoS ONE Lawrence, Kan : Public Library of Science 9 (3), p. 92474 1932-6203 BK010053 XK010053 XK010000 ETHRESEARCH ETHRESEARCH ETHRESEARCH Journal Article Open access