Handheld Lasers Allow Efficient Detection of Fluorescent Marked Organisms in the Field

Verfasser / Beitragende:
[Kevin B. Rice, Shelby J. Fleischer, Consuelo M. De Moraes, Mark C. Mescher, John F. Tooker, Moshe Gish]
Ort, Verlag, Jahr:
2015
Enthalten in:
PLoS ONE, 10 (6), p. e0129175
Format:
Artikel (online)
ID: 528783521
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024 7 0 |a 10.3929/ethz-b-000102226  |2 doi 
024 7 0 |a 10.1371/journal.pone.0129175  |2 doi 
035 |a (ETHRESEARCH)oai:www.research-collecti.ethz.ch:20.500.11850/102226 
245 0 0 |a Handheld Lasers Allow Efficient Detection of Fluorescent Marked Organisms in the Field  |h [Elektronische Daten]  |c [Kevin B. Rice, Shelby J. Fleischer, Consuelo M. De Moraes, Mark C. Mescher, John F. Tooker, Moshe Gish] 
246 0 |a PLoS ONE 
506 |a Open access  |2 ethresearch 
520 3 |a Marking organisms with fluorescent dyes and powders is a common technique used in ecological field studies that monitor movement of organisms to examine life history traits, behaviors, and population dynamics. External fluorescent marking is relatively inexpensive and can be readily employed to quickly mark large numbers of individuals; however, the ability to detect marked organisms in the field at night has been hampered by the limited detection distances provided by portable fluorescent ultraviolet lamps. In recent years, significant advances in LED lamp and laser technology have led to development of powerful, low-cost ultraviolet light sources. In this study, we evaluate the potential of these new technologies to improve detection of fluorescent-marked organisms in the field and to create new possibilities for tracking marked organisms in visually challenging environments such as tree canopies and aquatic habitats. Using handheld lasers, we document a method that provides a fivefold increase in detection distance over previously available technologies. This method allows easy scouting of tree canopies (from the ground), as well as shallow aquatic systems. This novel detection method for fluorescent-marked organisms thus promises to significantly enhance the use of fluorescent marking as a non-destructive technique for tracking organisms in natural environments, facilitating field studies that aim to document otherwise inaccessible aspects of the movement, behavior, and population dynamics of study organisms, including species with significant economic impacts or relevance for ecology and human health. 
540 |a Creative Commons Attribution 4.0 International  |u http://creativecommons.org/licenses/by/4.0  |2 ethresearch 
700 1 |a Rice  |D Kevin B.  |e joint author 
700 1 |a Fleischer  |D Shelby J.  |e joint author 
700 1 |a De Moraes  |D Consuelo M.  |e joint author 
700 1 |a Mescher  |D Mark C.  |e joint author 
700 1 |a Tooker  |D John F.  |e joint author 
700 1 |a Gish  |D Moshe  |e joint author 
773 0 |t PLoS ONE  |d San Francisco, CA : Public Library of Science  |g 10 (6), p. e0129175  |x 1932-6203 
856 4 0 |u http://hdl.handle.net/20.500.11850/102226  |q text/html  |z WWW-Backlink auf das Repository (Open access) 
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950 |B ETHRESEARCH  |P 856  |E 40  |u http://hdl.handle.net/20.500.11850/102226  |q text/html  |z WWW-Backlink auf das Repository (Open access) 
950 |B ETHRESEARCH  |P 700  |E 1-  |a Rice  |D Kevin B.  |e joint author 
950 |B ETHRESEARCH  |P 700  |E 1-  |a Fleischer  |D Shelby J.  |e joint author 
950 |B ETHRESEARCH  |P 700  |E 1-  |a De Moraes  |D Consuelo M.  |e joint author 
950 |B ETHRESEARCH  |P 700  |E 1-  |a Mescher  |D Mark C.  |e joint author 
950 |B ETHRESEARCH  |P 700  |E 1-  |a Tooker  |D John F.  |e joint author 
950 |B ETHRESEARCH  |P 700  |E 1-  |a Gish  |D Moshe  |e joint author 
950 |B ETHRESEARCH  |P 773  |E 0-  |t PLoS ONE  |d San Francisco, CA : Public Library of Science  |g 10 (6), p. e0129175  |x 1932-6203 
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