caa a22 4500 528784463 20190923032428.0 cr unu---uuuuu 180924e20110728xx s 000 0 eng 10.3929/ethz-b-000038573 doi 10.1371/journal.pone.0022459 doi (ETHRESEARCH)oai:www.research-collection.ethz.ch:20.500.11850/38573 Stromal IFN-γR-Signaling Modulates Goblet Cell Function During Salmonella Typhimurium Infection [Elektronische Daten] [Pascal Songhet, Manja Barthel, Bärbel Stecher, Andreas J. Müller, Markus Kremer, Gunnar C. Hansson, Wolf-Dietrich; id_orcid 0000-0002-9892-6420 Hardt] PLoS ONE Open access ethresearch Enteropathogenic bacteria are a frequent cause of diarrhea worldwide. The mucosal defenses against infection are not completely understood. We have used the streptomycin mouse model for Salmonella Typhimurium diarrhea to analyze the role of interferon gamma receptor (IFN-γR)-signaling in mucosal defense. IFN-γ is known to contribute to acute S. Typhimurium diarrhea. We have compared the acute mucosal inflammation in IFN-γR-/- mice and wild type animals. IFN-γR-/- mice harbored increased pathogen loads in the mucosal epithelium and the lamina propria. Surprisingly, the epithelium of the IFN-γR-/- mice did not show the dramatic "loss” of mucus-filled goblet cell vacuoles, a hallmark of the wild type mucosal infection. Using bone marrow chimeric mice we established that IFN-γR-signaling in stromal cells (e.g. goblet cells, enterocytes) controlled mucus excretion/vacuole loss by goblet cells. In contrast, IFN-γR-signaling in bone marrow-derived cells (e.g. macrophages, DCs, PMNs) was required for restricting pathogen growth in the gut tissue. Thus IFN-γR-signaling influences different mucosal responses to infection, including not only pathogen restriction in the lamina propria, but, as shown here, also goblet cell function. Creative Commons Attribution 3.0 Unported http://creativecommons.org/licenses/by/3.0 ethresearch Songhet Pascal joint author Barthel Manja joint author Stecher Bärbel joint author Müller Andreas J. joint author Kremer Markus joint author Hansson Gunnar C. joint author Hardt Wolf-Dietrich; id_orcid 0000-0002-9892-6420 joint author PLoS ONE Lawrence, KS, USA : Public Library of Science 6 (7), p. e22459 1932-6203 http://hdl.handle.net/20.500.11850/38573 text/html WWW-Backlink auf das Repository (Open access) 1 Journal Article ethresearch ETHRESEARCH 856 40 http://hdl.handle.net/20.500.11850/38573 text/html WWW-Backlink auf das Repository (Open access) ETHRESEARCH 700 1- Songhet Pascal joint author ETHRESEARCH 700 1- Barthel Manja joint author ETHRESEARCH 700 1- Stecher Bärbel joint author ETHRESEARCH 700 1- Müller Andreas J. joint author ETHRESEARCH 700 1- Kremer Markus joint author ETHRESEARCH 700 1- Hansson Gunnar C. joint author ETHRESEARCH 700 1- Hardt Wolf-Dietrich; id_orcid 0000-0002-9892-6420 joint author ETHRESEARCH 773 0- PLoS ONE Lawrence, KS, USA : Public Library of Science 6 (7), p. e22459 1932-6203 BK010053 XK010053 XK010000 ETHRESEARCH ETHRESEARCH ETHRESEARCH Journal Article Open access