αV-integrins are required for mechanotransduction in MDCK epithelial cells
| LEADER | naa a22 4500 | ||
|---|---|---|---|
| 001 | 528784854 | ||
| 005 | 20180924065517.0 | ||
| 007 | cr unu---uuuuu | ||
| 008 | 180924e20130819xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.3929/ethz-b-000282032 |2 doi |
| 024 | 7 | 0 | |a 10.1371/journal.pone.0071485 |2 doi |
| 035 | |a (ETHRESEARCH)oai:www.research-collecti.ethz.ch:20.500.11850/282032 | ||
| 245 | 0 | 0 | |a αV-integrins are required for mechanotransduction in MDCK epithelial cells |h [Elektronische Daten] |c [Terhi P. Teräväinen, Satu M. Myllymäki, Jens Friedrichs, Nico; id_orcid 0000-0002-5985-9800 Strohmeyer, Jose V. Moyano, Chuanyue Wu, Karl S. Matlin, Daniel J. Muller, Aki Manninen] |
| 246 | 0 | |a PLoS ONE | |
| 506 | |a Open access |2 ethresearch | ||
| 520 | 3 | |a The properties of epithelial cells within tissues are regulated by their immediate microenvironment, which consists of neighboring cells and the extracellular matrix (ECM). Integrin heterodimers orchestrate dynamic assembly and disassembly of cell-ECM connections and thereby convey biochemical and mechanical information from the ECM into cells. However, the specific contributions and functional hierarchy between different integrin heterodimers in the regulation of focal adhesion dynamics in epithelial cells are incompletely understood. Here, we have studied the functions of RGD-binding αV-integrins in a Madin Darby Canine Kidney (MDCK) cell model and found that αV-integrins regulate the maturation of focal adhesions (FAs) and cell spreading. αV-integrin-deficient MDCK cells bound collagen I (Col I) substrate via α2β1-integrins but failed to efficiently recruit FA components such as talin, focal adhesion kinase (FAK), vinculin and integrin-linked kinase (ILK). The apparent inability to mature α2β1-integrin-mediated FAs and link them to cellular actin cytoskeleton led to disrupted mechanotransduction in αV-integrin deficient cells seeded onto Col I substrate. | |
| 540 | |a Creative Commons Attribution 3.0 Unported |u http://creativecommons.org/licenses/by/3.0 |2 ethresearch | ||
| 700 | 1 | |a Teräväinen |D Terhi P. |e joint author | |
| 700 | 1 | |a Myllymäki |D Satu M. |e joint author | |
| 700 | 1 | |a Friedrichs |D Jens |e joint author | |
| 700 | 1 | |a Strohmeyer |D Nico; id_orcid 0000-0002-5985-9800 |e joint author | |
| 700 | 1 | |a Moyano |D Jose V. |e joint author | |
| 700 | 1 | |a Wu |D Chuanyue |e joint author | |
| 700 | 1 | |a Matlin |D Karl S. |e joint author | |
| 700 | 1 | |a Muller |D Daniel J. |e joint author | |
| 700 | 1 | |a Manninen |D Aki |e joint author | |
| 773 | 0 | |t PLoS ONE |d San Francisco, CA, USA : Public Library of Science |g 8 (8), p. e71485 |x 1932-6203 | |
| 856 | 4 | 0 | |u http://hdl.handle.net/20.500.11850/282032 |q text/html |z WWW-Backlink auf das Repository (Open access) |
| 908 | |D 1 |a Journal Article |2 ethresearch | ||
| 950 | |B ETHRESEARCH |P 856 |E 40 |u http://hdl.handle.net/20.500.11850/282032 |q text/html |z WWW-Backlink auf das Repository (Open access) | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Teräväinen |D Terhi P. |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Myllymäki |D Satu M. |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Friedrichs |D Jens |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Strohmeyer |D Nico; id_orcid 0000-0002-5985-9800 |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Moyano |D Jose V. |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Wu |D Chuanyue |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Matlin |D Karl S. |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Muller |D Daniel J. |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Manninen |D Aki |e joint author | ||
| 950 | |B ETHRESEARCH |P 773 |E 0- |t PLoS ONE |d San Francisco, CA, USA : Public Library of Science |g 8 (8), p. e71485 |x 1932-6203 | ||
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 949 | |B ETHRESEARCH |F ETHRESEARCH |b ETHRESEARCH |j Journal Article |c Open access | ||