naa a22 4500 528784854 20180924065517.0 cr unu---uuuuu 180924e20130819xx s 000 0 eng 10.3929/ethz-b-000282032 doi 10.1371/journal.pone.0071485 doi (ETHRESEARCH)oai:www.research-collection.ethz.ch:20.500.11850/282032 αV-integrins are required for mechanotransduction in MDCK epithelial cells [Elektronische Daten] [Terhi P. Teräväinen, Satu M. Myllymäki, Jens Friedrichs, Nico; id_orcid 0000-0002-5985-9800 Strohmeyer, Jose V. Moyano, Chuanyue Wu, Karl S. Matlin, Daniel J. Muller, Aki Manninen] PLoS ONE Open access ethresearch The properties of epithelial cells within tissues are regulated by their immediate microenvironment, which consists of neighboring cells and the extracellular matrix (ECM). Integrin heterodimers orchestrate dynamic assembly and disassembly of cell-ECM connections and thereby convey biochemical and mechanical information from the ECM into cells. However, the specific contributions and functional hierarchy between different integrin heterodimers in the regulation of focal adhesion dynamics in epithelial cells are incompletely understood. Here, we have studied the functions of RGD-binding αV-integrins in a Madin Darby Canine Kidney (MDCK) cell model and found that αV-integrins regulate the maturation of focal adhesions (FAs) and cell spreading. αV-integrin-deficient MDCK cells bound collagen I (Col I) substrate via α2β1-integrins but failed to efficiently recruit FA components such as talin, focal adhesion kinase (FAK), vinculin and integrin-linked kinase (ILK). The apparent inability to mature α2β1-integrin-mediated FAs and link them to cellular actin cytoskeleton led to disrupted mechanotransduction in αV-integrin deficient cells seeded onto Col I substrate. Creative Commons Attribution 3.0 Unported http://creativecommons.org/licenses/by/3.0 ethresearch Teräväinen Terhi P. joint author Myllymäki Satu M. joint author Friedrichs Jens joint author Strohmeyer Nico; id_orcid 0000-0002-5985-9800 joint author Moyano Jose V. joint author Wu Chuanyue joint author Matlin Karl S. joint author Muller Daniel J. joint author Manninen Aki joint author PLoS ONE San Francisco, CA, USA : Public Library of Science 8 (8), p. e71485 1932-6203 http://hdl.handle.net/20.500.11850/282032 text/html WWW-Backlink auf das Repository (Open access) 1 Journal Article ethresearch ETHRESEARCH 856 40 http://hdl.handle.net/20.500.11850/282032 text/html WWW-Backlink auf das Repository (Open access) ETHRESEARCH 700 1- Teräväinen Terhi P. joint author ETHRESEARCH 700 1- Myllymäki Satu M. joint author ETHRESEARCH 700 1- Friedrichs Jens joint author ETHRESEARCH 700 1- Strohmeyer Nico; id_orcid 0000-0002-5985-9800 joint author ETHRESEARCH 700 1- Moyano Jose V. joint author ETHRESEARCH 700 1- Wu Chuanyue joint author ETHRESEARCH 700 1- Matlin Karl S. joint author ETHRESEARCH 700 1- Muller Daniel J. joint author ETHRESEARCH 700 1- Manninen Aki joint author ETHRESEARCH 773 0- PLoS ONE San Francisco, CA, USA : Public Library of Science 8 (8), p. e71485 1932-6203 BK010053 XK010053 XK010000 ETHRESEARCH ETHRESEARCH ETHRESEARCH Journal Article Open access