caa a22 4500 528784935 20190923032428.0 cr unu---uuuuu 180924e20110609xx s 000 0 eng 10.3929/ethz-b-000037487 doi 10.1371/journal.pone.0020749 doi (ETHRESEARCH)oai:www.research-collection.ethz.ch:20.500.11850/37487 Salmonella-Induced Mucosal Lectin RegIIIβ Kills Competing Gut Microbiota [Elektronische Daten] [Christian Stelter, Rina Käppeli, Claudia König, Alexander Krah, Wolf-Dietrich; id_orcid 0000-0002-9892-6420 Hardt, Bärbel Stecher, Dirk Bumann] PLoS ONE Open access ethresearch Intestinal inflammation induces alterations of the gut microbiota and promotes overgrowth of the enteric pathogen Salmonella enterica by largely unknown mechanisms. Here, we identified a host factor involved in this process. Specifically, the C-type lectin RegIIIβ is strongly upregulated during mucosal infection and released into the gut lumen. In vitro, RegIIIβ kills diverse commensal gut bacteria but not Salmonella enterica subspecies I serovar Typhimurium (S. Typhimurium). Protection of the pathogen was attributable to its specific cell envelope structure. Co-infection experiments with an avirulent S. Typhimurium mutant and a RegIIIβ-sensitive commensal E. coli strain demonstrated that feeding of RegIIIβ was sufficient for suppressing commensals in the absence of all other changes inflicted by mucosal disease. These data suggest that RegIIIβ production by the host can promote S. Typhimurium infection by eliminating inhibitory gut microbiota. Creative Commons Attribution 3.0 Unported http://creativecommons.org/licenses/by/3.0 ethresearch Stelter Christian joint author Käppeli Rina joint author König Claudia joint author Krah Alexander joint author Hardt Wolf-Dietrich; id_orcid 0000-0002-9892-6420 joint author Stecher Bärbel joint author Bumann Dirk joint author PLoS ONE Lawrence, KS, USA : Public Library of Science 6 (6), p. e20749 1932-6203 http://hdl.handle.net/20.500.11850/37487 text/html WWW-Backlink auf das Repository (Open access) 1 Journal Article ethresearch ETHRESEARCH 856 40 http://hdl.handle.net/20.500.11850/37487 text/html WWW-Backlink auf das Repository (Open access) ETHRESEARCH 700 1- Stelter Christian joint author ETHRESEARCH 700 1- Käppeli Rina joint author ETHRESEARCH 700 1- König Claudia joint author ETHRESEARCH 700 1- Krah Alexander joint author ETHRESEARCH 700 1- Hardt Wolf-Dietrich; id_orcid 0000-0002-9892-6420 joint author ETHRESEARCH 700 1- Stecher Bärbel joint author ETHRESEARCH 700 1- Bumann Dirk joint author ETHRESEARCH 773 0- PLoS ONE Lawrence, KS, USA : Public Library of Science 6 (6), p. e20749 1932-6203 BK010053 XK010053 XK010000 ETHRESEARCH ETHRESEARCH ETHRESEARCH Journal Article Open access