naa a22 4500 528785109 20180924065518.0 cr unu---uuuuu 180924e20071212xx s 000 0 eng 10.3929/ethz-b-000004357 doi 10.1371/journal.pone.0001301 doi (ETHRESEARCH)oai:www.research-collection.ethz.ch:20.500.11850/4357 Revisiting Estimates of CTL Killing Rates In Vivo [Elektronische Daten] [Andrew Yates, Frederik Graw, Daniel L. Barber, Rafi Ahmed, Roland R. Regoes, Rustom Antia] PLoS ONE Open access ethresearch Recent experimental advances have allowed the estimation of the in vivo rates of killing of infected target cells by cytotoxic T lymphocytes (CTL). We present several refinements to a method applied previously to quantify killing of targets in the spleen using a dynamical model. We reanalyse data previously used to estimate killing rates of CTL specific for two epitopes of lymphocytic choriomeningitis virus (LCMV) in mice and show that, contrary to previous estimates the "killing rate” of effector CTL is approximately twice that of memory CTL. Further, our method allows the fits to be visualized, and reveals one potentially interesting discrepancy between fits and data. We discuss extensions to the basic CTL killing model to explain this discrepancy and propose experimental tests to distinguish between them. Creative Commons Attribution 3.0 Unported http://creativecommons.org/licenses/by/3.0 ethresearch Yates Andrew joint author Graw Frederik joint author Barber Daniel L. joint author Ahmed Rafi joint author Regoes Roland R. joint author Antia Rustom joint author PLoS ONE San Francisco, CA, USA : Public Library of Science 2 (12), p. e1301 1932-6203 http://hdl.handle.net/20.500.11850/4357 text/html WWW-Backlink auf das Repository (Open access) 1 Journal Article ethresearch ETHRESEARCH 856 40 http://hdl.handle.net/20.500.11850/4357 text/html WWW-Backlink auf das Repository (Open access) ETHRESEARCH 700 1- Yates Andrew joint author ETHRESEARCH 700 1- Graw Frederik joint author ETHRESEARCH 700 1- Barber Daniel L. joint author ETHRESEARCH 700 1- Ahmed Rafi joint author ETHRESEARCH 700 1- Regoes Roland R. joint author ETHRESEARCH 700 1- Antia Rustom joint author ETHRESEARCH 773 0- PLoS ONE San Francisco, CA, USA : Public Library of Science 2 (12), p. e1301 1932-6203 BK010053 XK010053 XK010000 ETHRESEARCH ETHRESEARCH ETHRESEARCH Journal Article Open access