caa a22 4500 528785389 20200103030208.0 cr unu---uuuuu 180924e201612 xx s 000 0 eng 10.3929/ethz-b-000124547 doi 10.7554/eLife.20640 doi (ETHRESEARCH)oai:www.research-collection.ethz.ch:20.500.11850/124547 Modularity and determinants of a (bi-)polarization control system from free-living and obligate intracellular bacteria [Elektronische Daten] [Matthieu Bergé, Sébastien Campagne, Johann Mignolet, Seamus Holden, Laurence Théraulaz, Suliana Manley, Frédéric H.-T. Allain, Patrick H. Viollier] Open access ethresearch Although free-living and obligate intracellular bacteria are both polarized it is unclear whether the underlying polarization mechanisms and effector proteins are conserved. Here we dissect at the cytological, functional and structural level a conserved polarization module from the free living α-proteobacterium Caulobacter crescentus and an orthologous system from an obligate intracellular (rickettsial) pathogen. The NMR solution structure of the zinc-finger (ZnR) domain from the bifunctional and bipolar ZitP pilus assembly/motility regulator revealed conserved interaction determinants for PopZ, a bipolar matrix protein that anchors the ParB centromere-binding protein and other regulatory factors at the poles. We show that ZitP regulates cytokinesis and the localization of ParB and PopZ, targeting PopZ independently of the previously known binding sites for its client proteins. Through heterologous localization assays with rickettsial ZitP and PopZ orthologs, we document the shared ancestries, activities and structural determinants of a (bi-)polarization system encoded in free-living and obligate intracellular α-proteobacteria. Creative Commons Attribution 4.0 International http://creativecommons.org/licenses/by/4.0 ethresearch Bergé Matthieu joint author Campagne Sébastien joint author Mignolet Johann joint author Holden Seamus joint author Théraulaz Laurence joint author Manley Suliana joint author Allain Frédéric H.-T joint author Viollier Patrick H. joint author eLife Cambridge : eLife Sciences Publishing 5, p. e20640 http://hdl.handle.net/20.500.11850/124547 text/html WWW-Backlink auf das Repository (Open access) 1 Journal Article ethresearch ETHRESEARCH 856 40 http://hdl.handle.net/20.500.11850/124547 text/html WWW-Backlink auf das Repository (Open access) ETHRESEARCH 700 1- Bergé Matthieu joint author ETHRESEARCH 700 1- Campagne Sébastien joint author ETHRESEARCH 700 1- Mignolet Johann joint author ETHRESEARCH 700 1- Holden Seamus joint author ETHRESEARCH 700 1- Théraulaz Laurence joint author ETHRESEARCH 700 1- Manley Suliana joint author ETHRESEARCH 700 1- Allain Frédéric H.-T joint author ETHRESEARCH 700 1- Viollier Patrick H. joint author ETHRESEARCH 773 0- eLife Cambridge : eLife Sciences Publishing 5, p. e20640 BK010053 XK010053 XK010000 ETHRESEARCH ETHRESEARCH ETHRESEARCH Journal Article Open access