c-di-GMP heterogeneity is generated by the chemotaxis machinery to regulate flagellar motility
| LEADER | caa a22 4500 | ||
|---|---|---|---|
| 001 | 528785400 | ||
| 005 | 20190908091347.0 | ||
| 007 | cr unu---uuuuu | ||
| 008 | 180924e201312 xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.3929/ethz-b-000078240 |2 doi |
| 024 | 7 | 0 | |a 10.7554/eLife.01402.001 |2 doi |
| 035 | |a (ETHRESEARCH)oai:www.research-collecti.ethz.ch:20.500.11850/78240 | ||
| 245 | 0 | 0 | |a c-di-GMP heterogeneity is generated by the chemotaxis machinery to regulate flagellar motility |h [Elektronische Daten] |c [Bridget R. Kulasekara, Cassandra Kamischke, Hemantha D. Kulasekara, Matthias Christen, Paul A. Wiggins, Samuel I. Miller] |
| 506 | |a Open access |2 ethresearch | ||
| 520 | 3 | |a Individual cell heterogeneity is commonly observed within populations, although its molecular basis is largely unknown. Previously, using FRET-based microscopy, we observed heterogeneity in cellular c-di-GMP levels. In this study, we show that c-di-GMP heterogeneity in Pseudomonas aeruginosa is promoted by a specific phosphodiesterase partitioned after cell division. We found that subcellular localization and reduction of c-di-GMP levels by this phosphodiesterase is dependent on the histidine kinase component of the chemotaxis machinery, CheA, and its phosphorylation state. Therefore, individual cell heterogeneity in c-di-GMP concentrations is regulated by the activity and the asymmetrical inheritance of the chemotaxis organelle after cell division. c-di-GMP heterogeneity results in a diversity of motility behaviors. The generation of diverse intracellular concentrations of c-di-GMP by asymmetric partitioning is likely important to the success and survival of bacterial populations within the environment by allowing a variety of motility behaviors. | |
| 540 | |a Creative Commons Attribution 3.0 Unported |u http://creativecommons.org/licenses/by/3.0 |2 ethresearch | ||
| 700 | 1 | |a Kulasekara |D Bridget R. |e joint author | |
| 700 | 1 | |a Kamischke |D Cassandra |e joint author | |
| 700 | 1 | |a Kulasekara |D Hemantha D. |e joint author | |
| 700 | 1 | |a Christen |D Matthias |e joint author | |
| 700 | 1 | |a Wiggins |D Paul A. |e joint author | |
| 700 | 1 | |a Miller |D Samuel I. |e joint author | |
| 773 | 0 | |t eLife |d Cambridge : eLife Sciences Publications |g 2, p. e01402 |x 2050-084X | |
| 856 | 4 | 0 | |u http://hdl.handle.net/20.500.11850/78240 |q text/html |z WWW-Backlink auf das Repository (Open access) |
| 908 | |D 1 |a Journal Article |2 ethresearch | ||
| 950 | |B ETHRESEARCH |P 856 |E 40 |u http://hdl.handle.net/20.500.11850/78240 |q text/html |z WWW-Backlink auf das Repository (Open access) | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Kulasekara |D Bridget R. |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Kamischke |D Cassandra |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Kulasekara |D Hemantha D. |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Christen |D Matthias |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Wiggins |D Paul A. |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Miller |D Samuel I. |e joint author | ||
| 950 | |B ETHRESEARCH |P 773 |E 0- |t eLife |d Cambridge : eLife Sciences Publications |g 2, p. e01402 |x 2050-084X | ||
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 949 | |B ETHRESEARCH |F ETHRESEARCH |b ETHRESEARCH |j Journal Article |c Open access | ||