caa a22 4500 528785400 20190908091347.0 cr unu---uuuuu 180924e201312 xx s 000 0 eng 10.3929/ethz-b-000078240 doi 10.7554/eLife.01402.001 doi (ETHRESEARCH)oai:www.research-collection.ethz.ch:20.500.11850/78240 c-di-GMP heterogeneity is generated by the chemotaxis machinery to regulate flagellar motility [Elektronische Daten] [Bridget R. Kulasekara, Cassandra Kamischke, Hemantha D. Kulasekara, Matthias Christen, Paul A. Wiggins, Samuel I. Miller] Open access ethresearch Individual cell heterogeneity is commonly observed within populations, although its molecular basis is largely unknown. Previously, using FRET-based microscopy, we observed heterogeneity in cellular c-di-GMP levels. In this study, we show that c-di-GMP heterogeneity in Pseudomonas aeruginosa is promoted by a specific phosphodiesterase partitioned after cell division. We found that subcellular localization and reduction of c-di-GMP levels by this phosphodiesterase is dependent on the histidine kinase component of the chemotaxis machinery, CheA, and its phosphorylation state. Therefore, individual cell heterogeneity in c-di-GMP concentrations is regulated by the activity and the asymmetrical inheritance of the chemotaxis organelle after cell division. c-di-GMP heterogeneity results in a diversity of motility behaviors. The generation of diverse intracellular concentrations of c-di-GMP by asymmetric partitioning is likely important to the success and survival of bacterial populations within the environment by allowing a variety of motility behaviors. Creative Commons Attribution 3.0 Unported http://creativecommons.org/licenses/by/3.0 ethresearch Kulasekara Bridget R. joint author Kamischke Cassandra joint author Kulasekara Hemantha D. joint author Christen Matthias joint author Wiggins Paul A. joint author Miller Samuel I. joint author eLife Cambridge : eLife Sciences Publications 2, p. e01402 2050-084X http://hdl.handle.net/20.500.11850/78240 text/html WWW-Backlink auf das Repository (Open access) 1 Journal Article ethresearch ETHRESEARCH 856 40 http://hdl.handle.net/20.500.11850/78240 text/html WWW-Backlink auf das Repository (Open access) ETHRESEARCH 700 1- Kulasekara Bridget R. joint author ETHRESEARCH 700 1- Kamischke Cassandra joint author ETHRESEARCH 700 1- Kulasekara Hemantha D. joint author ETHRESEARCH 700 1- Christen Matthias joint author ETHRESEARCH 700 1- Wiggins Paul A. joint author ETHRESEARCH 700 1- Miller Samuel I. joint author ETHRESEARCH 773 0- eLife Cambridge : eLife Sciences Publications 2, p. e01402 2050-084X BK010053 XK010053 XK010000 ETHRESEARCH ETHRESEARCH ETHRESEARCH Journal Article Open access