caa a22 4500 52878546X 20181217030312.0 cr unu---uuuuu 180924e20130813xx s 000 0 eng 10.3929/ethz-b-000071142 doi 10.1038/srep02425 doi (ETHRESEARCH)oai:www.research-collection.ethz.ch:20.500.11850/71142 Mesenchymal Stem Cells Exploit Extracellular Matrix as Mechanotransducer [Elektronische Daten] [Bojun Li, Cameron Moshfegh, Zhe Lin, Jörg Albuschies, Viola Vogel] Sci Rep Open access ethresearch While stem cells can sense and respond to physical properties of their environment, the molecular aspects how physical information is translated into biochemical signals remain unknown. Here we show that human mesenchymal stem cells (hMSCs) harvest and assemble plasma fibronectin into their extracellular matrix (ECM) fibrils within 24 hours. hMSCs pro-actively pull on newly assembled fibronectin ECM fibrils, and the fibers are more stretched on rigid than on soft fibronectin-coated polyacrylamide gels. Culturing hMSCs on single stretched fibronectin fibers upregulates hMSC osteogenesis. Osteogenesis was increased when αvβ3 integrins were blocked on relaxed fibronectin fibers, and decreased when α5β1 integrins were blocked or when epidermal growth factor (EGF) receptor signaling was inhibited on stretched fibronectin fibers. This suggests that hMSCs utilize their own contractile forces to translate environmental cues into differential biochemical signals by stretching fibronectin fibrils. Mechanoregulation of fibronectin fibrils may thus serve as check point to regulate hMSC osteogenesis. Creative Commons Attribution 3.0 Unported http://creativecommons.org/licenses/by/3.0 ethresearch Biophysics ethresearch Biotechnology ethresearch Cell Biology ethresearch Stem Cells ethresearch Subject Areas ethresearch Li Bojun joint author Moshfegh Cameron joint author Lin Zhe joint author Albuschies Jörg joint author Vogel Viola joint author Scientific Reports London : Nature Publishing Group 3, p. 2425 2045-2322 http://hdl.handle.net/20.500.11850/71142 text/html WWW-Backlink auf das Repository (Open access) 1 Journal Article ethresearch ETHRESEARCH 856 40 http://hdl.handle.net/20.500.11850/71142 text/html WWW-Backlink auf das Repository (Open access) ETHRESEARCH 700 1- Li Bojun joint author ETHRESEARCH 700 1- Moshfegh Cameron joint author ETHRESEARCH 700 1- Lin Zhe joint author ETHRESEARCH 700 1- Albuschies Jörg joint author ETHRESEARCH 700 1- Vogel Viola joint author ETHRESEARCH 773 0- Scientific Reports London : Nature Publishing Group 3, p. 2425 2045-2322 BK010053 XK010053 XK010000 ETHRESEARCH ETHRESEARCH ETHRESEARCH Journal Article Open access