caa a22 4500 528785583 20190908091347.0 cr unu---uuuuu 180924e201507 xx s 000 0 eng 10.3929/ethz-b-000107549 doi 10.7554/eLife.07661 doi (ETHRESEARCH)oai:www.research-collection.ethz.ch:20.500.11850/107549 An open-source computational and data resource to analyze digital maps of immunopeptidomes [Elektronische Daten] [Etienne Caron, Lucia Espona, Daniel J. Kowalewski, Heiko Schuster, Nicola Ternette, Adán Alpízar, Ralf B. Schittenhelm, Sri H. Ramarathinam, Cecilia S. Lindestam Arlehamn, Ching Chiek Koh, Ludovic; id_orcid 0000-0002-1001-3265 Gillet, Armin Rabsteyn, Pedro Navarro, Sangtae Kim, Henry Lam, Theo Sturm, Miguel Marcilla, Alessandro Sette, David S. Campbell, Eric W. Deutsch, Robert L. Moritz, Anthony W. Purcell, Hans-Georg Rammensee, Stefan Stevanović, Ruedi Aebersold] Open access ethresearch We present a novel mass spectrometry-based high-throughput workflow and an open-source computational and data resource to reproducibly identify and quantify HLA-associated peptides. Collectively, the resources support the generation of HLA allele-specific peptide assay libraries consisting of consensus fragment ion spectra, and the analysis of quantitative digital maps of HLA peptidomes generated from a range of biological sources by SWATH mass spectrometry (MS). This study represents the first community-based effort to develop a robust platform for the reproducible and quantitative measurement of the entire repertoire of peptides presented by HLA molecules, an essential step towards the design of efficient immunotherapies. Creative Commons Attribution 4.0 International http://creativecommons.org/licenses/by/4.0 ethresearch Caron Etienne joint author Espona Lucia joint author Kowalewski Daniel J. joint author Schuster Heiko joint author Ternette Nicola joint author Alpízar Adán joint author Schittenhelm Ralf B. joint author Ramarathinam Sri H. joint author Lindestam Arlehamn Cecilia S. joint author Koh Ching Chiek joint author Gillet Ludovic; id_orcid 0000-0002-1001-3265 joint author Rabsteyn Armin joint author Navarro Pedro joint author Kim Sangtae joint author Lam Henry joint author Sturm Theo joint author Marcilla Miguel joint author Sette Alessandro joint author Campbell David S. joint author Deutsch Eric W. joint author Moritz Robert L. joint author Purcell Anthony W. joint author Rammensee Hans-Georg joint author Stevanović Stefan joint author Aebersold Ruedi joint author eLife Cambridge : eLife Sciences Publications 4, p. e07661 2050-084X http://hdl.handle.net/20.500.11850/107549 text/html WWW-Backlink auf das Repository (Open access) 1 Journal Article ethresearch ETHRESEARCH 856 40 http://hdl.handle.net/20.500.11850/107549 text/html WWW-Backlink auf das Repository (Open access) ETHRESEARCH 700 1- Caron Etienne joint author ETHRESEARCH 700 1- Espona Lucia joint author ETHRESEARCH 700 1- Kowalewski Daniel J. joint author ETHRESEARCH 700 1- Schuster Heiko joint author ETHRESEARCH 700 1- Ternette Nicola joint author ETHRESEARCH 700 1- Alpízar Adán joint author ETHRESEARCH 700 1- Schittenhelm Ralf B. joint author ETHRESEARCH 700 1- Ramarathinam Sri H. joint author ETHRESEARCH 700 1- Lindestam Arlehamn Cecilia S. joint author ETHRESEARCH 700 1- Koh Ching Chiek joint author ETHRESEARCH 700 1- Gillet Ludovic; id_orcid 0000-0002-1001-3265 joint author ETHRESEARCH 700 1- Rabsteyn Armin joint author ETHRESEARCH 700 1- Navarro Pedro joint author ETHRESEARCH 700 1- Kim Sangtae joint author ETHRESEARCH 700 1- Lam Henry joint author ETHRESEARCH 700 1- Sturm Theo joint author ETHRESEARCH 700 1- Marcilla Miguel joint author ETHRESEARCH 700 1- Sette Alessandro joint author ETHRESEARCH 700 1- Campbell David S. joint author ETHRESEARCH 700 1- Deutsch Eric W. joint author ETHRESEARCH 700 1- Moritz Robert L. joint author ETHRESEARCH 700 1- Purcell Anthony W. joint author ETHRESEARCH 700 1- Rammensee Hans-Georg joint author ETHRESEARCH 700 1- Stevanović Stefan joint author ETHRESEARCH 700 1- Aebersold Ruedi joint author ETHRESEARCH 773 0- eLife Cambridge : eLife Sciences Publications 4, p. e07661 2050-084X BK010053 XK010053 XK010000 ETHRESEARCH ETHRESEARCH ETHRESEARCH Journal Article Open access