Impact of ribonucleotide incorporation by DNA polymerases β and λ on oxidative base excision repair
| LEADER | caa a22 4500 | ||
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| 001 | 528785702 | ||
| 005 | 20190713030954.0 | ||
| 007 | cr unu---uuuuu | ||
| 008 | 180924s2016 xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.3929/ethz-b-000113967 |2 doi |
| 024 | 7 | 0 | |a 10.1038/ncomms10805 |2 doi |
| 035 | |a (ETHRESEARCH)oai:www.research-collecti.ethz.ch:20.500.11850/113967 | ||
| 245 | 0 | 0 | |a Impact of ribonucleotide incorporation by DNA polymerases β and λ on oxidative base excision repair |h [Elektronische Daten] |c [Emmanuele Crespan, Antonia Furrer, Marcel Rösinger, Federica Bertoletti, Elisa Mentegari, Giulia Chiapparini, Ralph Imhof, Nathalie Ziegler, Shana J.; id_orcid 0000-0001-6808-5950 Sturla, Ulrich Hübscher, Barbara Van Loon, Giovanni Maga] |
| 246 | 0 | |a Nat Commun | |
| 506 | |a Open access |2 ethresearch | ||
| 520 | 3 | |a Oxidative stress is a very frequent source of DNA damage. Many cellular DNA polymerases (Pols) can incorporate ribonucleotides (rNMPs) during DNA synthesis. However, whether oxidative stress-triggered DNA repair synthesis contributes to genomic rNMPs incorporation is so far not fully understood. Human specialized Pols β and λ are the important enzymes involved in the oxidative stress tolerance, acting both in base excision repair and in translesion synthesis past the very frequent oxidative lesion 7,8-dihydro-8-oxoguanine (8-oxo-G). We found that Pol β, to a greater extent than Pol λ can incorporate rNMPs opposite normal bases or 8-oxo-G, and with a different fidelity. Further, the incorporation of rNMPs opposite 8-oxo-G delays repair by DNA glycosylases. Studies in Pol β- and λ-deficient cell extracts suggest that Pol β levels can greatly affect rNMP incorporation opposite oxidative DNA lesions. | |
| 540 | |a Creative Commons Attribution 4.0 International |u http://creativecommons.org/licenses/by/4.0 |2 ethresearch | ||
| 700 | 1 | |a Crespan |D Emmanuele |e joint author | |
| 700 | 1 | |a Furrer |D Antonia |e joint author | |
| 700 | 1 | |a Rösinger |D Marcel |e joint author | |
| 700 | 1 | |a Bertoletti |D Federica |e joint author | |
| 700 | 1 | |a Mentegari |D Elisa |e joint author | |
| 700 | 1 | |a Chiapparini |D Giulia |e joint author | |
| 700 | 1 | |a Imhof |D Ralph |e joint author | |
| 700 | 1 | |a Ziegler |D Nathalie |e joint author | |
| 700 | 1 | |a Sturla |D Shana J.; id_orcid 0000-0001-6808-5950 |e joint author | |
| 700 | 1 | |a Hübscher |D Ulrich |e joint author | |
| 700 | 1 | |a Van Loon |D Barbara |e joint author | |
| 700 | 1 | |a Maga |D Giovanni |e joint author | |
| 773 | 0 | |t Nature Communications |d London : Nature Publishing Group |g 7, p. 10805 |x 2041-1723 | |
| 856 | 4 | 0 | |u http://hdl.handle.net/20.500.11850/113967 |q text/html |z WWW-Backlink auf das Repository (Open access) |
| 908 | |D 1 |a Journal Article |2 ethresearch | ||
| 950 | |B ETHRESEARCH |P 856 |E 40 |u http://hdl.handle.net/20.500.11850/113967 |q text/html |z WWW-Backlink auf das Repository (Open access) | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Crespan |D Emmanuele |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Furrer |D Antonia |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Rösinger |D Marcel |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Bertoletti |D Federica |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Mentegari |D Elisa |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Chiapparini |D Giulia |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Imhof |D Ralph |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Ziegler |D Nathalie |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Sturla |D Shana J.; id_orcid 0000-0001-6808-5950 |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Hübscher |D Ulrich |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Van Loon |D Barbara |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Maga |D Giovanni |e joint author | ||
| 950 | |B ETHRESEARCH |P 773 |E 0- |t Nature Communications |d London : Nature Publishing Group |g 7, p. 10805 |x 2041-1723 | ||
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 949 | |B ETHRESEARCH |F ETHRESEARCH |b ETHRESEARCH |j Journal Article |c Open access | ||