caa a22 4500 528785702 20190713030954.0 cr unu---uuuuu 180924s2016 xx s 000 0 eng 10.3929/ethz-b-000113967 doi 10.1038/ncomms10805 doi (ETHRESEARCH)oai:www.research-collection.ethz.ch:20.500.11850/113967 Impact of ribonucleotide incorporation by DNA polymerases β and λ on oxidative base excision repair [Elektronische Daten] [Emmanuele Crespan, Antonia Furrer, Marcel Rösinger, Federica Bertoletti, Elisa Mentegari, Giulia Chiapparini, Ralph Imhof, Nathalie Ziegler, Shana J.; id_orcid 0000-0001-6808-5950 Sturla, Ulrich Hübscher, Barbara Van Loon, Giovanni Maga] Nat Commun Open access ethresearch Oxidative stress is a very frequent source of DNA damage. Many cellular DNA polymerases (Pols) can incorporate ribonucleotides (rNMPs) during DNA synthesis. However, whether oxidative stress-triggered DNA repair synthesis contributes to genomic rNMPs incorporation is so far not fully understood. Human specialized Pols β and λ are the important enzymes involved in the oxidative stress tolerance, acting both in base excision repair and in translesion synthesis past the very frequent oxidative lesion 7,8-dihydro-8-oxoguanine (8-oxo-G). We found that Pol β, to a greater extent than Pol λ can incorporate rNMPs opposite normal bases or 8-oxo-G, and with a different fidelity. Further, the incorporation of rNMPs opposite 8-oxo-G delays repair by DNA glycosylases. Studies in Pol β- and λ-deficient cell extracts suggest that Pol β levels can greatly affect rNMP incorporation opposite oxidative DNA lesions. Creative Commons Attribution 4.0 International http://creativecommons.org/licenses/by/4.0 ethresearch Crespan Emmanuele joint author Furrer Antonia joint author Rösinger Marcel joint author Bertoletti Federica joint author Mentegari Elisa joint author Chiapparini Giulia joint author Imhof Ralph joint author Ziegler Nathalie joint author Sturla Shana J.; id_orcid 0000-0001-6808-5950 joint author Hübscher Ulrich joint author Van Loon Barbara joint author Maga Giovanni joint author Nature Communications London : Nature Publishing Group 7, p. 10805 2041-1723 http://hdl.handle.net/20.500.11850/113967 text/html WWW-Backlink auf das Repository (Open access) 1 Journal Article ethresearch ETHRESEARCH 856 40 http://hdl.handle.net/20.500.11850/113967 text/html WWW-Backlink auf das Repository (Open access) ETHRESEARCH 700 1- Crespan Emmanuele joint author ETHRESEARCH 700 1- Furrer Antonia joint author ETHRESEARCH 700 1- Rösinger Marcel joint author ETHRESEARCH 700 1- Bertoletti Federica joint author ETHRESEARCH 700 1- Mentegari Elisa joint author ETHRESEARCH 700 1- Chiapparini Giulia joint author ETHRESEARCH 700 1- Imhof Ralph joint author ETHRESEARCH 700 1- Ziegler Nathalie joint author ETHRESEARCH 700 1- Sturla Shana J.; id_orcid 0000-0001-6808-5950 joint author ETHRESEARCH 700 1- Hübscher Ulrich joint author ETHRESEARCH 700 1- Van Loon Barbara joint author ETHRESEARCH 700 1- Maga Giovanni joint author ETHRESEARCH 773 0- Nature Communications London : Nature Publishing Group 7, p. 10805 2041-1723 BK010053 XK010053 XK010000 ETHRESEARCH ETHRESEARCH ETHRESEARCH Journal Article Open access