Molecular architecture of native fibronectin fibrils
| LEADER | caa a22 4500 | ||
|---|---|---|---|
| 001 | 528785842 | ||
| 005 | 20190713030955.0 | ||
| 007 | cr unu---uuuuu | ||
| 008 | 180924s2015 xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.3929/ethz-b-000101332 |2 doi |
| 024 | 7 | 0 | |a 10.1038/ncomms8275 |2 doi |
| 035 | |a (ETHRESEARCH)oai:www.research-collecti.ethz.ch:20.500.11850/101332 | ||
| 245 | 0 | 0 | |a Molecular architecture of native fibronectin fibrils |h [Elektronische Daten] |c [Susanna M. Früh, Ingmar Schoen, Jonas Ries, Viola Vogel] |
| 246 | 0 | |a Nat Commun | |
| 506 | |a Open access |2 ethresearch | ||
| 520 | 3 | |a Fibronectin fibrils within the extracellular matrix play central roles in physiological and pathological processes, yet many structural details about their hierarchical and molecular assembly remain unknown. Here we combine site-specific protein labelling with single-molecule localization by stepwise photobleaching or direct stochastic optical reconstruction microscopy (dSTORM), and determine the relative positions of various labelled sites within native matrix fibrils. Single end-labelled fibronectin molecules in fibrils display an average end-to-end distance of ∼133 nm. Sampling of site-specific antibody epitopes along the thinnest fibrils (protofibrils) shows periodic punctate label patterns with ∼95 nm repeats and alternating N- and C-terminal regions. These measurements suggest an antiparallel 30-40 nm overlap between N-termini, suggesting that the first five type I modules bind type III modules of the adjacent molecule. Thicker fibres show random bundling of protofibrils without a well-defined line-up. This super-resolution microscopy approach can be applied to other fibrillar protein assemblies of unknown structure. | |
| 540 | |a Creative Commons Attribution 4.0 International |u http://creativecommons.org/licenses/by/4.0 |2 ethresearch | ||
| 700 | 1 | |a Früh |D Susanna M. |e joint author | |
| 700 | 1 | |a Schoen |D Ingmar |e joint author | |
| 700 | 1 | |a Ries |D Jonas |e joint author | |
| 700 | 1 | |a Vogel |D Viola |e joint author | |
| 773 | 0 | |t Nature Communications |d London : Nature Publishing Group |g 6, p. 7275 |x 2041-1723 | |
| 856 | 4 | 0 | |u http://hdl.handle.net/20.500.11850/101332 |q text/html |z WWW-Backlink auf das Repository (Open access) |
| 908 | |D 1 |a Journal Article |2 ethresearch | ||
| 950 | |B ETHRESEARCH |P 856 |E 40 |u http://hdl.handle.net/20.500.11850/101332 |q text/html |z WWW-Backlink auf das Repository (Open access) | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Früh |D Susanna M. |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Schoen |D Ingmar |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Ries |D Jonas |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Vogel |D Viola |e joint author | ||
| 950 | |B ETHRESEARCH |P 773 |E 0- |t Nature Communications |d London : Nature Publishing Group |g 6, p. 7275 |x 2041-1723 | ||
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 949 | |B ETHRESEARCH |F ETHRESEARCH |b ETHRESEARCH |j Journal Article |c Open access | ||