caa a22 4500 528785842 20190713030955.0 cr unu---uuuuu 180924s2015 xx s 000 0 eng 10.3929/ethz-b-000101332 doi 10.1038/ncomms8275 doi (ETHRESEARCH)oai:www.research-collection.ethz.ch:20.500.11850/101332 Molecular architecture of native fibronectin fibrils [Elektronische Daten] [Susanna M. Früh, Ingmar Schoen, Jonas Ries, Viola Vogel] Nat Commun Open access ethresearch Fibronectin fibrils within the extracellular matrix play central roles in physiological and pathological processes, yet many structural details about their hierarchical and molecular assembly remain unknown. Here we combine site-specific protein labelling with single-molecule localization by stepwise photobleaching or direct stochastic optical reconstruction microscopy (dSTORM), and determine the relative positions of various labelled sites within native matrix fibrils. Single end-labelled fibronectin molecules in fibrils display an average end-to-end distance of ∼133 nm. Sampling of site-specific antibody epitopes along the thinnest fibrils (protofibrils) shows periodic punctate label patterns with ∼95 nm repeats and alternating N- and C-terminal regions. These measurements suggest an antiparallel 30-40 nm overlap between N-termini, suggesting that the first five type I modules bind type III modules of the adjacent molecule. Thicker fibres show random bundling of protofibrils without a well-defined line-up. This super-resolution microscopy approach can be applied to other fibrillar protein assemblies of unknown structure. Creative Commons Attribution 4.0 International http://creativecommons.org/licenses/by/4.0 ethresearch Früh Susanna M. joint author Schoen Ingmar joint author Ries Jonas joint author Vogel Viola joint author Nature Communications London : Nature Publishing Group 6, p. 7275 2041-1723 http://hdl.handle.net/20.500.11850/101332 text/html WWW-Backlink auf das Repository (Open access) 1 Journal Article ethresearch ETHRESEARCH 856 40 http://hdl.handle.net/20.500.11850/101332 text/html WWW-Backlink auf das Repository (Open access) ETHRESEARCH 700 1- Früh Susanna M. joint author ETHRESEARCH 700 1- Schoen Ingmar joint author ETHRESEARCH 700 1- Ries Jonas joint author ETHRESEARCH 700 1- Vogel Viola joint author ETHRESEARCH 773 0- Nature Communications London : Nature Publishing Group 6, p. 7275 2041-1723 BK010053 XK010053 XK010000 ETHRESEARCH ETHRESEARCH ETHRESEARCH Journal Article Open access