Mechanical forces regulate the interactions of fibronectin and collagen I in extracellular matrix
| LEADER | caa a22 4500 | ||
|---|---|---|---|
| 001 | 528785893 | ||
| 005 | 20190713030955.0 | ||
| 007 | cr unu---uuuuu | ||
| 008 | 180924s2015 xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.3929/ethz-b-000103188 |2 doi |
| 024 | 7 | 0 | |a 10.1038/ncomms9026 |2 doi |
| 035 | |a (ETHRESEARCH)oai:www.research-collecti.ethz.ch:20.500.11850/103188 | ||
| 245 | 0 | 0 | |a Mechanical forces regulate the interactions of fibronectin and collagen I in extracellular matrix |h [Elektronische Daten] |c [Kristopher E. Kubow, Radmila Vukmirovic, Zhe Lin, Enrico; id_orcid 0000-0002-7577-9042 Klotzsch, Michael L. Smith, Delphine Gourdon, Sheila Luna, Viola Vogel] |
| 246 | 0 | |a Nat Commun | |
| 506 | |a Open access |2 ethresearch | ||
| 520 | 3 | |a Despite the crucial role of extracellular matrix (ECM) in directing cell fate in healthy and diseased tissues—particularly in development, wound healing, tissue regeneration and cancer—the mechanisms that direct the assembly and regulate hierarchical architectures of ECM are poorly understood. Collagen I matrix assembly in vivo requires active fibronectin (Fn) fibrillogenesis by cells. Here we exploit Fn-FRET probes as mechanical strain sensors and demonstrate that collagen I fibres preferentially co-localize with more-relaxed Fn fibrils in the ECM of fibroblasts in cell culture. Fibre stretch-assay studies reveal that collagen I's Fn-binding domain is responsible for the mechano-regulated interaction. Furthermore, we show that Fn-collagen interactions are reciprocal: relaxed Fn fibrils act as multivalent templates for collagen assembly, but once assembled, collagen fibres shield Fn fibres from being stretched by cellular traction forces. Thus, in addition to the well-recognized, force-regulated, cell-matrix interactions, forces also tune the interactions between different structural ECM components. | |
| 540 | |a Creative Commons Attribution 4.0 International |u http://creativecommons.org/licenses/by/4.0 |2 ethresearch | ||
| 700 | 1 | |a Kubow |D Kristopher E. |e joint author | |
| 700 | 1 | |a Vukmirovic |D Radmila |e joint author | |
| 700 | 1 | |a Lin |D Zhe |e joint author | |
| 700 | 1 | |a Klotzsch |D Enrico; id_orcid 0000-0002-7577-9042 |e joint author | |
| 700 | 1 | |a Smith |D Michael L. |e joint author | |
| 700 | 1 | |a Gourdon |D Delphine |e joint author | |
| 700 | 1 | |a Luna |D Sheila |e joint author | |
| 700 | 1 | |a Vogel |D Viola |e joint author | |
| 773 | 0 | |t Nature Communications |d London : Nature Publishing Group |g 6, p. 8026 |x 2041-1723 | |
| 856 | 4 | 0 | |u http://hdl.handle.net/20.500.11850/103188 |q text/html |z WWW-Backlink auf das Repository (Open access) |
| 908 | |D 1 |a Journal Article |2 ethresearch | ||
| 950 | |B ETHRESEARCH |P 856 |E 40 |u http://hdl.handle.net/20.500.11850/103188 |q text/html |z WWW-Backlink auf das Repository (Open access) | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Kubow |D Kristopher E. |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Vukmirovic |D Radmila |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Lin |D Zhe |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Klotzsch |D Enrico; id_orcid 0000-0002-7577-9042 |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Smith |D Michael L. |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Gourdon |D Delphine |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Luna |D Sheila |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Vogel |D Viola |e joint author | ||
| 950 | |B ETHRESEARCH |P 773 |E 0- |t Nature Communications |d London : Nature Publishing Group |g 6, p. 8026 |x 2041-1723 | ||
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 949 | |B ETHRESEARCH |F ETHRESEARCH |b ETHRESEARCH |j Journal Article |c Open access | ||