caa a22 4500 528785893 20190713030955.0 cr unu---uuuuu 180924s2015 xx s 000 0 eng 10.3929/ethz-b-000103188 doi 10.1038/ncomms9026 doi (ETHRESEARCH)oai:www.research-collection.ethz.ch:20.500.11850/103188 Mechanical forces regulate the interactions of fibronectin and collagen I in extracellular matrix [Elektronische Daten] [Kristopher E. Kubow, Radmila Vukmirovic, Zhe Lin, Enrico; id_orcid 0000-0002-7577-9042 Klotzsch, Michael L. Smith, Delphine Gourdon, Sheila Luna, Viola Vogel] Nat Commun Open access ethresearch Despite the crucial role of extracellular matrix (ECM) in directing cell fate in healthy and diseased tissues—particularly in development, wound healing, tissue regeneration and cancer—the mechanisms that direct the assembly and regulate hierarchical architectures of ECM are poorly understood. Collagen I matrix assembly in vivo requires active fibronectin (Fn) fibrillogenesis by cells. Here we exploit Fn-FRET probes as mechanical strain sensors and demonstrate that collagen I fibres preferentially co-localize with more-relaxed Fn fibrils in the ECM of fibroblasts in cell culture. Fibre stretch-assay studies reveal that collagen I's Fn-binding domain is responsible for the mechano-regulated interaction. Furthermore, we show that Fn-collagen interactions are reciprocal: relaxed Fn fibrils act as multivalent templates for collagen assembly, but once assembled, collagen fibres shield Fn fibres from being stretched by cellular traction forces. Thus, in addition to the well-recognized, force-regulated, cell-matrix interactions, forces also tune the interactions between different structural ECM components. Creative Commons Attribution 4.0 International http://creativecommons.org/licenses/by/4.0 ethresearch Kubow Kristopher E. joint author Vukmirovic Radmila joint author Lin Zhe joint author Klotzsch Enrico; id_orcid 0000-0002-7577-9042 joint author Smith Michael L. joint author Gourdon Delphine joint author Luna Sheila joint author Vogel Viola joint author Nature Communications London : Nature Publishing Group 6, p. 8026 2041-1723 http://hdl.handle.net/20.500.11850/103188 text/html WWW-Backlink auf das Repository (Open access) 1 Journal Article ethresearch ETHRESEARCH 856 40 http://hdl.handle.net/20.500.11850/103188 text/html WWW-Backlink auf das Repository (Open access) ETHRESEARCH 700 1- Kubow Kristopher E. joint author ETHRESEARCH 700 1- Vukmirovic Radmila joint author ETHRESEARCH 700 1- Lin Zhe joint author ETHRESEARCH 700 1- Klotzsch Enrico; id_orcid 0000-0002-7577-9042 joint author ETHRESEARCH 700 1- Smith Michael L. joint author ETHRESEARCH 700 1- Gourdon Delphine joint author ETHRESEARCH 700 1- Luna Sheila joint author ETHRESEARCH 700 1- Vogel Viola joint author ETHRESEARCH 773 0- Nature Communications London : Nature Publishing Group 6, p. 8026 2041-1723 BK010053 XK010053 XK010000 ETHRESEARCH ETHRESEARCH ETHRESEARCH Journal Article Open access