The biomechanical properties of an epithelial tissue determine the location of its vasculature
| LEADER | caa a22 4500 | ||
|---|---|---|---|
| 001 | 528785982 | ||
| 005 | 20190713030955.0 | ||
| 007 | cr unu---uuuuu | ||
| 008 | 180924s2016 xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.3929/ethz-b-000124146 |2 doi |
| 024 | 7 | 0 | |a 10.1038/ncomms13560 |2 doi |
| 035 | |a (ETHRESEARCH)oai:www.research-collecti.ethz.ch:20.500.11850/124146 | ||
| 245 | 0 | 4 | |a The biomechanical properties of an epithelial tissue determine the location of its vasculature |h [Elektronische Daten] |c [Martin Kragl, Rajib Schubert, Haiko Karsjens, Silke Otter, Barbara Bartosinska, Kay Jeruschke, Jürgen Weiss, Chunguang Chen, David Alsteens, Oliver Kuss, Stephan Speier, Daniel Eberhard, Daniel J.; id_orcid 0000-0003-3075-0665 Müller, Eckhard Lammert] |
| 246 | 0 | |a Nat Commun | |
| 506 | |a Open access |2 ethresearch | ||
| 520 | 3 | |a An important question is how growing tissues establish a blood vessel network. Here we study vascular network formation in pancreatic islets, endocrine tissues derived from pancreatic epithelium. We find that depletion of integrin-linked kinase (ILK) in the pancreatic epithelial cells of mice results in glucose intolerance due to a loss of the intra-islet vasculature. In turn, blood vessels accumulate at the islet periphery. Neither alterations in endothelial cell proliferation, apoptosis, morphology, Vegfa expression and VEGF-A secretion nor ‘empty sleeves' of vascular basement membrane are found. Instead, biophysical experiments reveal that the biomechanical properties of pancreatic islet cells, such as their actomyosin-mediated cortex tension and adhesive forces to endothelial cells, are significantly changed. These results suggest that a sorting event is driving the segregation of endothelial and epithelial cells and indicate that the epithelial biomechanical properties determine whether the blood vasculature invades or envelops a growing epithelial tissue. | |
| 540 | |a Creative Commons Attribution 4.0 International |u http://creativecommons.org/licenses/by/4.0 |2 ethresearch | ||
| 700 | 1 | |a Kragl |D Martin |e joint author | |
| 700 | 1 | |a Schubert |D Rajib |e joint author | |
| 700 | 1 | |a Karsjens |D Haiko |e joint author | |
| 700 | 1 | |a Otter |D Silke |e joint author | |
| 700 | 1 | |a Bartosinska |D Barbara |e joint author | |
| 700 | 1 | |a Jeruschke |D Kay |e joint author | |
| 700 | 1 | |a Weiss |D Jürgen |e joint author | |
| 700 | 1 | |a Chen |D Chunguang |e joint author | |
| 700 | 1 | |a Alsteens |D David |e joint author | |
| 700 | 1 | |a Kuss |D Oliver |e joint author | |
| 700 | 1 | |a Speier |D Stephan |e joint author | |
| 700 | 1 | |a Eberhard |D Daniel |e joint author | |
| 700 | 1 | |a Müller |D Daniel J.; id_orcid 0000-0003-3075-0665 |e joint author | |
| 700 | 1 | |a Lammert |D Eckhard |e joint author | |
| 773 | 0 | |t Nature Communications |d London : Nature Publishing Group |g 7, p. 13560 |x 2041-1723 | |
| 856 | 4 | 0 | |u http://hdl.handle.net/20.500.11850/124146 |q text/html |z WWW-Backlink auf das Repository (Open access) |
| 908 | |D 1 |a Journal Article |2 ethresearch | ||
| 950 | |B ETHRESEARCH |P 856 |E 40 |u http://hdl.handle.net/20.500.11850/124146 |q text/html |z WWW-Backlink auf das Repository (Open access) | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Kragl |D Martin |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Schubert |D Rajib |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Karsjens |D Haiko |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Otter |D Silke |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Bartosinska |D Barbara |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Jeruschke |D Kay |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Weiss |D Jürgen |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Chen |D Chunguang |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Alsteens |D David |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Kuss |D Oliver |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Speier |D Stephan |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Eberhard |D Daniel |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Müller |D Daniel J.; id_orcid 0000-0003-3075-0665 |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Lammert |D Eckhard |e joint author | ||
| 950 | |B ETHRESEARCH |P 773 |E 0- |t Nature Communications |d London : Nature Publishing Group |g 7, p. 13560 |x 2041-1723 | ||
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 949 | |B ETHRESEARCH |F ETHRESEARCH |b ETHRESEARCH |j Journal Article |c Open access | ||