caa a22 4500 528786261 20181217030314.0 cr unu---uuuuu 180924e20140606xx s 000 0 eng 10.3929/ethz-b-000084899 doi 10.1038/srep05196 doi (ETHRESEARCH)oai:www.research-collection.ethz.ch:20.500.11850/84899 Fingerprinting Breast Cancer vs. Normal Mammary Cells by Mass Spectrometric Analysis of Volatiles [Elektronische Daten] [Jingjing He, Pablo M.-L. Sinues, Maija Hollmén, Xue Li, Michael Detmar, Renato; id_orcid 0000-0001-5211-4358 Zenobi] Sci Rep Open access ethresearch There is increasing interest in the development of noninvasive diagnostic methods for early cancer detection, to improve the survival rate and quality of life of cancer patients. Identification of volatile metabolic compounds may provide an approach for noninvasive early diagnosis of malignant diseases. Here we analyzed the volatile metabolic signature of human breast cancer cell lines versus normal human mammary cells. Volatile compounds in the headspace of conditioned culture medium were directly fingerprinted by secondary electrospray ionization-mass spectrometry. The mass spectra were subsequently treated statistically to identify discriminating features between normal vs. cancerous cell types. We were able to classify different samples by using feature selection followed by principal component analysis (PCA). Additionally, high-resolution mass spectrometry allowed us to propose their chemical structures for some of the most discriminating molecules. We conclude that cancerous cells can release a characteristic odor whose constituents may be used as disease markers. Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported http://creativecommons.org/licenses/by-nc-sa/3.0 ethresearch Mass spectrometry ethresearch Diagnostic markers ethresearch He Jingjing joint author Sinues Pablo M.-L joint author Hollmén Maija joint author Li Xue joint author Detmar Michael joint author Zenobi Renato; id_orcid 0000-0001-5211-4358 joint author Scientific Reports London : Nature Publishing Group 4, p. 5196 2045-2322 http://hdl.handle.net/20.500.11850/84899 text/html WWW-Backlink auf das Repository (Open access) 1 Journal Article ethresearch ETHRESEARCH 856 40 http://hdl.handle.net/20.500.11850/84899 text/html WWW-Backlink auf das Repository (Open access) ETHRESEARCH 700 1- He Jingjing joint author ETHRESEARCH 700 1- Sinues Pablo M.-L joint author ETHRESEARCH 700 1- Hollmén Maija joint author ETHRESEARCH 700 1- Li Xue joint author ETHRESEARCH 700 1- Detmar Michael joint author ETHRESEARCH 700 1- Zenobi Renato; id_orcid 0000-0001-5211-4358 joint author ETHRESEARCH 773 0- Scientific Reports London : Nature Publishing Group 4, p. 5196 2045-2322 BK010053 XK010053 XK010000 ETHRESEARCH ETHRESEARCH ETHRESEARCH Journal Article Open access