Mind-controlled transgene expression by a wireless-powered optogenetic designer cell implant
| LEADER | caa a22 4500 | ||
|---|---|---|---|
| 001 | 52878627X | ||
| 005 | 20190713030956.0 | ||
| 007 | cr unu---uuuuu | ||
| 008 | 180924s2014 xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.3929/ethz-b-000094958 |2 doi |
| 024 | 7 | 0 | |a 10.1038/ncomms6392 |2 doi |
| 035 | |a (ETHRESEARCH)oai:www.research-collecti.ethz.ch:20.500.11850/94958 | ||
| 245 | 0 | 0 | |a Mind-controlled transgene expression by a wireless-powered optogenetic designer cell implant |h [Elektronische Daten] |c [Marc Folcher, Sabine Oesterle, Katharina Zwicky, Thushara Thekkottil, Julie Heymoz, Muriel Hohmann, Matthias Christen, Marie D. El-Baba, Peter Buchmann, Martin Fussenegger] |
| 246 | 0 | |a Nat Commun | |
| 506 | |a Open access |2 ethresearch | ||
| 520 | 3 | |a Synthetic devices for traceless remote control of gene expression may provide new treatment opportunities in future gene- and cell-based therapies. Here we report the design of a synthetic mind-controlled gene switch that enables human brain activities and mental states to wirelessly programme the transgene expression in human cells. An electroencephalography (EEG)-based brain-computer interface (BCI) processing mental state-specific brain waves programs an inductively linked wireless-powered optogenetic implant containing designer cells engineered for near-infrared (NIR) light-adjustable expression of the human glycoprotein SEAP (secreted alkaline phosphatase). The synthetic optogenetic signalling pathway interfacing the BCI with target gene expression consists of an engineered NIR light-activated bacterial diguanylate cyclase (DGCL) producing the orthogonal second messenger cyclic diguanosine monophosphate (c-di-GMP), which triggers the stimulator of interferon genes (STING)-dependent induction of synthetic interferon-β promoters. Humans generating different mental states (biofeedback control, concentration, meditation) can differentially control SEAP production of the designer cells in culture and of subcutaneous wireless-powered optogenetic implants in mice. | |
| 540 | |a Creative Commons Attribution 4.0 International |u http://creativecommons.org/licenses/by/4.0 |2 ethresearch | ||
| 700 | 1 | |a Folcher |D Marc |e joint author | |
| 700 | 1 | |a Oesterle |D Sabine |e joint author | |
| 700 | 1 | |a Zwicky |D Katharina |e joint author | |
| 700 | 1 | |a Thekkottil |D Thushara |e joint author | |
| 700 | 1 | |a Heymoz |D Julie |e joint author | |
| 700 | 1 | |a Hohmann |D Muriel |e joint author | |
| 700 | 1 | |a Christen |D Matthias |e joint author | |
| 700 | 1 | |a El-Baba |D Marie D. |e joint author | |
| 700 | 1 | |a Buchmann |D Peter |e joint author | |
| 700 | 1 | |a Fussenegger |D Martin |e joint author | |
| 773 | 0 | |t Nature Communications |d London : Nature Publishing Group |g 5, p. 5392 |x 2041-1723 | |
| 856 | 4 | 0 | |u http://hdl.handle.net/20.500.11850/94958 |q text/html |z WWW-Backlink auf das Repository (Open access) |
| 908 | |D 1 |a Journal Article |2 ethresearch | ||
| 950 | |B ETHRESEARCH |P 856 |E 40 |u http://hdl.handle.net/20.500.11850/94958 |q text/html |z WWW-Backlink auf das Repository (Open access) | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Folcher |D Marc |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Oesterle |D Sabine |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Zwicky |D Katharina |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Thekkottil |D Thushara |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Heymoz |D Julie |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Hohmann |D Muriel |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Christen |D Matthias |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a El-Baba |D Marie D. |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Buchmann |D Peter |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Fussenegger |D Martin |e joint author | ||
| 950 | |B ETHRESEARCH |P 773 |E 0- |t Nature Communications |d London : Nature Publishing Group |g 5, p. 5392 |x 2041-1723 | ||
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 949 | |B ETHRESEARCH |F ETHRESEARCH |b ETHRESEARCH |j Journal Article |c Open access | ||