caa a22 4500 52878627X 20190713030956.0 cr unu---uuuuu 180924s2014 xx s 000 0 eng 10.3929/ethz-b-000094958 doi 10.1038/ncomms6392 doi (ETHRESEARCH)oai:www.research-collection.ethz.ch:20.500.11850/94958 Mind-controlled transgene expression by a wireless-powered optogenetic designer cell implant [Elektronische Daten] [Marc Folcher, Sabine Oesterle, Katharina Zwicky, Thushara Thekkottil, Julie Heymoz, Muriel Hohmann, Matthias Christen, Marie D. El-Baba, Peter Buchmann, Martin Fussenegger] Nat Commun Open access ethresearch Synthetic devices for traceless remote control of gene expression may provide new treatment opportunities in future gene- and cell-based therapies. Here we report the design of a synthetic mind-controlled gene switch that enables human brain activities and mental states to wirelessly programme the transgene expression in human cells. An electroencephalography (EEG)-based brain-computer interface (BCI) processing mental state-specific brain waves programs an inductively linked wireless-powered optogenetic implant containing designer cells engineered for near-infrared (NIR) light-adjustable expression of the human glycoprotein SEAP (secreted alkaline phosphatase). The synthetic optogenetic signalling pathway interfacing the BCI with target gene expression consists of an engineered NIR light-activated bacterial diguanylate cyclase (DGCL) producing the orthogonal second messenger cyclic diguanosine monophosphate (c-di-GMP), which triggers the stimulator of interferon genes (STING)-dependent induction of synthetic interferon-β promoters. Humans generating different mental states (biofeedback control, concentration, meditation) can differentially control SEAP production of the designer cells in culture and of subcutaneous wireless-powered optogenetic implants in mice. Creative Commons Attribution 4.0 International http://creativecommons.org/licenses/by/4.0 ethresearch Folcher Marc joint author Oesterle Sabine joint author Zwicky Katharina joint author Thekkottil Thushara joint author Heymoz Julie joint author Hohmann Muriel joint author Christen Matthias joint author El-Baba Marie D. joint author Buchmann Peter joint author Fussenegger Martin joint author Nature Communications London : Nature Publishing Group 5, p. 5392 2041-1723 http://hdl.handle.net/20.500.11850/94958 text/html WWW-Backlink auf das Repository (Open access) 1 Journal Article ethresearch ETHRESEARCH 856 40 http://hdl.handle.net/20.500.11850/94958 text/html WWW-Backlink auf das Repository (Open access) ETHRESEARCH 700 1- Folcher Marc joint author ETHRESEARCH 700 1- Oesterle Sabine joint author ETHRESEARCH 700 1- Zwicky Katharina joint author ETHRESEARCH 700 1- Thekkottil Thushara joint author ETHRESEARCH 700 1- Heymoz Julie joint author ETHRESEARCH 700 1- Hohmann Muriel joint author ETHRESEARCH 700 1- Christen Matthias joint author ETHRESEARCH 700 1- El-Baba Marie D. joint author ETHRESEARCH 700 1- Buchmann Peter joint author ETHRESEARCH 700 1- Fussenegger Martin joint author ETHRESEARCH 773 0- Nature Communications London : Nature Publishing Group 5, p. 5392 2041-1723 BK010053 XK010053 XK010000 ETHRESEARCH ETHRESEARCH ETHRESEARCH Journal Article Open access