Synthetic vanillate-regulated promoter for graded gene expression in Sphingomonas
| LEADER | caa a22 4500 | ||
|---|---|---|---|
| 001 | 528786288 | ||
| 005 | 20181217030314.0 | ||
| 007 | cr unu---uuuuu | ||
| 008 | 180924e20140929xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.3929/ethz-b-000090618 |2 doi |
| 024 | 7 | 0 | |a 10.1038/srep06453 |2 doi |
| 035 | |a (ETHRESEARCH)oai:www.research-collecti.ethz.ch:20.500.11850/90618 | ||
| 100 | 1 | |a Kaczmarczyk |D Andreas | |
| 245 | 1 | 0 | |a Synthetic vanillate-regulated promoter for graded gene expression in Sphingomonas |h [Elektronische Daten] |c [Andreas Kaczmarczyk, Julia A. Vorholt, Anne Francez-Charlot] |
| 246 | 0 | |a Sci Rep | |
| 506 | |a Open access |2 ethresearch | ||
| 520 | 3 | |a Regulated promoters are an important basic genetic tool allowing, for example, gene-dosage and gene depletion studies. We have previously described a cumate-inducible promoter (PQ5) that is functional in diverse Alphaproteobacteria. This promoter has been engineered by combining a synthetic minimal promoter, Psyn2, and operator sites and the repressor of the Pseudomonas putida F1 cym/cmt system. In the present study, we engineered a vanillate-regulated promoter using Psyn2 and the regulatory elements of the Caulobacter crescentus vanR-vanAB system. We show that the resulting promoter, which we called PV10, responds rapidly to the inducer vanillate with an induction ratio of about two orders of magnitude in Sphingomonas melonis Fr1. In contrast to the switch-like behavior of PQ5, PV10 shows a linear dose-response curve at intermediate vanillate concentrations, allowing graded gene expression. PV10 is functionally compatible with and independent of PQ5 and cumate, and vice versa, suggesting that both systems can be used simultaneously. | |
| 540 | |a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International |u http://creativecommons.org/licenses/by-nc-nd/4.0 |2 ethresearch | ||
| 690 | 7 | |a Bacterial techniques and applications |2 ethresearch | |
| 690 | 7 | |a Bacterial genes |2 ethresearch | |
| 700 | 1 | |a Vorholt |D Julia A. |e joint author | |
| 700 | 1 | |a Francez-Charlot |D Anne |e joint author | |
| 773 | 0 | |t Scientific Reports |d London : Nature Publishing Group |g 4, p. 6453 |x 2045-2322 | |
| 856 | 4 | 0 | |u http://hdl.handle.net/20.500.11850/90618 |q text/html |z WWW-Backlink auf das Repository (Open access) |
| 908 | |D 1 |a Journal Article |2 ethresearch | ||
| 950 | |B ETHRESEARCH |P 856 |E 40 |u http://hdl.handle.net/20.500.11850/90618 |q text/html |z WWW-Backlink auf das Repository (Open access) | ||
| 950 | |B ETHRESEARCH |P 100 |E 1- |a Kaczmarczyk |D Andreas | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Vorholt |D Julia A. |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Francez-Charlot |D Anne |e joint author | ||
| 950 | |B ETHRESEARCH |P 773 |E 0- |t Scientific Reports |d London : Nature Publishing Group |g 4, p. 6453 |x 2045-2322 | ||
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 949 | |B ETHRESEARCH |F ETHRESEARCH |b ETHRESEARCH |j Journal Article |c Open access | ||