CD24 tracks divergent pluripotent states in mouse and human cells
| LEADER | caa a22 4500 | ||
|---|---|---|---|
| 001 | 528786296 | ||
| 005 | 20190713030956.0 | ||
| 007 | cr unu---uuuuu | ||
| 008 | 180924s2015 xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.3929/ethz-b-000102532 |2 doi |
| 024 | 7 | 0 | |a 10.1038/ncomms8329 |2 doi |
| 035 | |a (ETHRESEARCH)oai:www.research-collecti.ethz.ch:20.500.11850/102532 | ||
| 245 | 0 | 0 | |a CD24 tracks divergent pluripotent states in mouse and human cells |h [Elektronische Daten] |c [Nika Shakiba, Carl A. White, Yonatan Y. Lipsitz, Ayako Yachie-Kinoshita, Peter D. Tonge, Samer M.I. Hussein, Mira C. Puri, Judith Elbaz, James Morrissey-Scoot, Mira Li, Javier Munoz, Marco Benevento, Ian M. Rogers, Jacob H. Hanna, Albert J.R. Heck, Bernd Wollscheid, Andras Nagy, Peter W. Zandstra] |
| 246 | 0 | |a Nat Commun | |
| 506 | |a Open access |2 ethresearch | ||
| 520 | 3 | |a Reprogramming is a dynamic process that can result in multiple pluripotent cell types emerging from divergent paths. Cell surface protein expression is a particularly desirable tool to categorize reprogramming and pluripotency as it enables robust quantification and enrichment of live cells. Here we use cell surface proteomics to interrogate mouse cell reprogramming dynamics and discover CD24 as a marker that tracks the emergence of reprogramming-responsive cells, while enabling the analysis and enrichment of transgene-dependent (F-class) and -independent (traditional) induced pluripotent stem cells (iPSCs) at later stages. Furthermore, CD24 can be used to delineate epiblast stem cells (EpiSCs) from embryonic stem cells (ESCs) in mouse pluripotent culture. Importantly, regulated CD24 expression is conserved in human pluripotent stem cells (PSCs), tracking the conversion of human ESCs to more naive-like PSC states. Thus, CD24 is a conserved marker for tracking divergent states in both reprogramming and standard pluripotent culture. | |
| 540 | |a Creative Commons Attribution 4.0 International |u http://creativecommons.org/licenses/by/4.0 |2 ethresearch | ||
| 700 | 1 | |a Shakiba |D Nika |e joint author | |
| 700 | 1 | |a White |D Carl A. |e joint author | |
| 700 | 1 | |a Lipsitz |D Yonatan Y. |e joint author | |
| 700 | 1 | |a Yachie-Kinoshita |D Ayako |e joint author | |
| 700 | 1 | |a Tonge |D Peter D. |e joint author | |
| 700 | 1 | |a Hussein |D Samer M.I. |e joint author | |
| 700 | 1 | |a Puri |D Mira C. |e joint author | |
| 700 | 1 | |a Elbaz |D Judith |e joint author | |
| 700 | 1 | |a Morrissey-Scoot |D James |e joint author | |
| 700 | 1 | |a Li |D Mira |e joint author | |
| 700 | 1 | |a Munoz |D Javier |e joint author | |
| 700 | 1 | |a Benevento |D Marco |e joint author | |
| 700 | 1 | |a Rogers |D Ian M. |e joint author | |
| 700 | 1 | |a Hanna |D Jacob H. |e joint author | |
| 700 | 1 | |a Heck |D Albert J.R. |e joint author | |
| 700 | 1 | |a Wollscheid |D Bernd |e joint author | |
| 700 | 1 | |a Nagy |D Andras |e joint author | |
| 700 | 1 | |a Zandstra |D Peter W. |e joint author | |
| 773 | 0 | |t Nature Communications |d London : Nature Publishing Group |g 6, p. 7329 |x 2041-1723 | |
| 856 | 4 | 0 | |u http://hdl.handle.net/20.500.11850/102532 |q text/html |z WWW-Backlink auf das Repository (Open access) |
| 908 | |D 1 |a Journal Article |2 ethresearch | ||
| 950 | |B ETHRESEARCH |P 856 |E 40 |u http://hdl.handle.net/20.500.11850/102532 |q text/html |z WWW-Backlink auf das Repository (Open access) | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Shakiba |D Nika |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a White |D Carl A. |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Lipsitz |D Yonatan Y. |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Yachie-Kinoshita |D Ayako |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Tonge |D Peter D. |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Hussein |D Samer M.I. |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Puri |D Mira C. |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Elbaz |D Judith |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Morrissey-Scoot |D James |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Li |D Mira |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Munoz |D Javier |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Benevento |D Marco |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Rogers |D Ian M. |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Hanna |D Jacob H. |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Heck |D Albert J.R. |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Wollscheid |D Bernd |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Nagy |D Andras |e joint author | ||
| 950 | |B ETHRESEARCH |P 700 |E 1- |a Zandstra |D Peter W. |e joint author | ||
| 950 | |B ETHRESEARCH |P 773 |E 0- |t Nature Communications |d London : Nature Publishing Group |g 6, p. 7329 |x 2041-1723 | ||
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 949 | |B ETHRESEARCH |F ETHRESEARCH |b ETHRESEARCH |j Journal Article |c Open access | ||