caa a22 4500 528786296 20190713030956.0 cr unu---uuuuu 180924s2015 xx s 000 0 eng 10.3929/ethz-b-000102532 doi 10.1038/ncomms8329 doi (ETHRESEARCH)oai:www.research-collection.ethz.ch:20.500.11850/102532 CD24 tracks divergent pluripotent states in mouse and human cells [Elektronische Daten] [Nika Shakiba, Carl A. White, Yonatan Y. Lipsitz, Ayako Yachie-Kinoshita, Peter D. Tonge, Samer M.I. Hussein, Mira C. Puri, Judith Elbaz, James Morrissey-Scoot, Mira Li, Javier Munoz, Marco Benevento, Ian M. Rogers, Jacob H. Hanna, Albert J.R. Heck, Bernd Wollscheid, Andras Nagy, Peter W. Zandstra] Nat Commun Open access ethresearch Reprogramming is a dynamic process that can result in multiple pluripotent cell types emerging from divergent paths. Cell surface protein expression is a particularly desirable tool to categorize reprogramming and pluripotency as it enables robust quantification and enrichment of live cells. Here we use cell surface proteomics to interrogate mouse cell reprogramming dynamics and discover CD24 as a marker that tracks the emergence of reprogramming-responsive cells, while enabling the analysis and enrichment of transgene-dependent (F-class) and -independent (traditional) induced pluripotent stem cells (iPSCs) at later stages. Furthermore, CD24 can be used to delineate epiblast stem cells (EpiSCs) from embryonic stem cells (ESCs) in mouse pluripotent culture. Importantly, regulated CD24 expression is conserved in human pluripotent stem cells (PSCs), tracking the conversion of human ESCs to more naive-like PSC states. Thus, CD24 is a conserved marker for tracking divergent states in both reprogramming and standard pluripotent culture. Creative Commons Attribution 4.0 International http://creativecommons.org/licenses/by/4.0 ethresearch Shakiba Nika joint author White Carl A. joint author Lipsitz Yonatan Y. joint author Yachie-Kinoshita Ayako joint author Tonge Peter D. joint author Hussein Samer M.I. joint author Puri Mira C. joint author Elbaz Judith joint author Morrissey-Scoot James joint author Li Mira joint author Munoz Javier joint author Benevento Marco joint author Rogers Ian M. joint author Hanna Jacob H. joint author Heck Albert J.R. joint author Wollscheid Bernd joint author Nagy Andras joint author Zandstra Peter W. joint author Nature Communications London : Nature Publishing Group 6, p. 7329 2041-1723 http://hdl.handle.net/20.500.11850/102532 text/html WWW-Backlink auf das Repository (Open access) 1 Journal Article ethresearch ETHRESEARCH 856 40 http://hdl.handle.net/20.500.11850/102532 text/html WWW-Backlink auf das Repository (Open access) ETHRESEARCH 700 1- Shakiba Nika joint author ETHRESEARCH 700 1- White Carl A. joint author ETHRESEARCH 700 1- Lipsitz Yonatan Y. joint author ETHRESEARCH 700 1- Yachie-Kinoshita Ayako joint author ETHRESEARCH 700 1- Tonge Peter D. joint author ETHRESEARCH 700 1- Hussein Samer M.I. joint author ETHRESEARCH 700 1- Puri Mira C. joint author ETHRESEARCH 700 1- Elbaz Judith joint author ETHRESEARCH 700 1- Morrissey-Scoot James joint author ETHRESEARCH 700 1- Li Mira joint author ETHRESEARCH 700 1- Munoz Javier joint author ETHRESEARCH 700 1- Benevento Marco joint author ETHRESEARCH 700 1- Rogers Ian M. joint author ETHRESEARCH 700 1- Hanna Jacob H. joint author ETHRESEARCH 700 1- Heck Albert J.R. joint author ETHRESEARCH 700 1- Wollscheid Bernd joint author ETHRESEARCH 700 1- Nagy Andras joint author ETHRESEARCH 700 1- Zandstra Peter W. joint author ETHRESEARCH 773 0- Nature Communications London : Nature Publishing Group 6, p. 7329 2041-1723 BK010053 XK010053 XK010000 ETHRESEARCH ETHRESEARCH ETHRESEARCH Journal Article Open access