caa a22 4500 52878630X 20190713030956.0 cr unu---uuuuu 180924s2015 xx s 000 0 eng 10.3929/ethz-b-000102533 doi 10.1038/ncomms8305 doi (ETHRESEARCH)oai:www.research-collection.ethz.ch:20.500.11850/102533 A network comprising short and long noncoding RNAs and RNA helicase controls mouse retina architecture [Elektronische Daten] [Jacek Krol, Ilona Krol, Claudia P. Patino Alvarez, Michele Fiscella, Andreas; id_orcid 0000-0002-3838-2468 Hierlemann, Botond Roska, Witold Filipowicz] Nat Commun Open access ethresearch Brain regions, such as the cortex and retina, are composed of layers of uniform thickness. The molecular mechanism that controls this uniformity is not well understood. Here we show that during mouse postnatal development the timed expression of Rncr4, a retina-specific long noncoding RNA, regulates the similarly timed processing of pri-miR-183/96/182, which is repressed at an earlier developmental stage by RNA helicase Ddx3x. Shifting the timing of mature miR-183/96/182 accumulation or interfering with Ddx3x expression leads to the disorganization of retinal architecture, with the photoreceptor layer being most affected. We identify Crb1, a component of the adhesion belt between glial and photoreceptor cells, as a link between Rncr4-regulated miRNA metabolism and uniform retina layering. Our results suggest that the precise timing of glia-neuron interaction controlled by noncoding RNAs and Ddx3x is important for the even distribution of cells across layers. Creative Commons Attribution 4.0 International http://creativecommons.org/licenses/by/4.0 ethresearch Krol Jacek joint author Krol Ilona joint author Patino Alvarez Claudia P. joint author Fiscella Michele joint author Hierlemann Andreas; id_orcid 0000-0002-3838-2468 joint author Roska Botond joint author Filipowicz Witold joint author Nature Communications London : Nature Publishing Group 6, p. 7305 2041-1723 http://hdl.handle.net/20.500.11850/102533 text/html WWW-Backlink auf das Repository (Open access) 1 Journal Article ethresearch ETHRESEARCH 856 40 http://hdl.handle.net/20.500.11850/102533 text/html WWW-Backlink auf das Repository (Open access) ETHRESEARCH 700 1- Krol Jacek joint author ETHRESEARCH 700 1- Krol Ilona joint author ETHRESEARCH 700 1- Patino Alvarez Claudia P. joint author ETHRESEARCH 700 1- Fiscella Michele joint author ETHRESEARCH 700 1- Hierlemann Andreas; id_orcid 0000-0002-3838-2468 joint author ETHRESEARCH 700 1- Roska Botond joint author ETHRESEARCH 700 1- Filipowicz Witold joint author ETHRESEARCH 773 0- Nature Communications London : Nature Publishing Group 6, p. 7305 2041-1723 BK010053 XK010053 XK010000 ETHRESEARCH ETHRESEARCH ETHRESEARCH Journal Article Open access